Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5199223..5199848 Replicon chromosome
Accession NZ_CP045694
Organism Klebsiella pneumoniae strain TK421

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag GHA50_RS26355 Protein ID WP_002882817.1
Coordinates 5199223..5199606 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag GHA50_RS26360 Protein ID WP_004150355.1
Coordinates 5199606..5199848 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GHA50_RS26340 5196589..5197491 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
GHA50_RS26345 5197488..5198123 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
GHA50_RS26350 5198120..5199049 + 930 WP_153459467.1 formate dehydrogenase accessory protein FdhE -
GHA50_RS26355 5199223..5199606 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
GHA50_RS26360 5199606..5199848 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
GHA50_RS26365 5200053..5200970 + 918 WP_004895173.1 alpha/beta hydrolase -
GHA50_RS26370 5200984..5201925 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
GHA50_RS26375 5201970..5202407 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
GHA50_RS26380 5202404..5203264 - 861 WP_023280688.1 virulence factor BrkB family protein -
GHA50_RS26385 5203258..5203857 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T142305 WP_002882817.1 NZ_CP045694:c5199606-5199223 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T142305 NZ_CP060647:1548116-1548451 [Streptococcus pyogenes]
TTGGACTATAAGAAATATCAGATTATCTATGCTCCTGATGTTTTAGAGAAGCTAAAAGAAATTCGTGATTATATTTCTCA
AAACTACTCCTCAACATCAGGTCAGCGTAAAATGGAGCAAATCATTAGCGACATAGAAAAACTCGAGGTTTTCCCAGAAG
TTGGTTTTGATGCCGATGAAAAATATGGTTCAAAAATCAGCCATTATCACAGCACTAAAGGTTACACCTTAAGTAAAGAT
TATATTGTCCTATACCATATCGAGGGGGAAGAAAATAGGGTCGCTATTGATTACTTGCTTCCTACTCAAAGCGACTATAT
AAAGTTATTCAAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT142305 WP_004150355.1 NZ_CP045694:c5199848-5199606 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT142305 NZ_CP060647:1547839-1548126 [Streptococcus pyogenes]
ATGACTACAGTAAAAAAAAACAGAGCGGTTACCTTTCAAGCTAATAAAGAATTGGTAAGCGAAGCAATGACAGTATTAAA
CAAAAAAAATTTAACCTTATCATCTGCTTTAAGATTATTCCTACAAAATGTCGTTGTCACAAACGAGGTTGACTTATTGA
CGGAAGAGGAGTTAGAGAAAGAAAAACTTTTCAAGCAATTTCAAGCTGAAATCAACAAAAATATTGAGGACGTTCGTCAA
GGGAAGTTTTATACCTCTGAAGAAGTGAGGGCTGAACTTGGACTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References