Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4034057..4034676 Replicon chromosome
Accession NZ_CP045694
Organism Klebsiella pneumoniae strain TK421

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag GHA50_RS20890 Protein ID WP_002892050.1
Coordinates 4034458..4034676 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag GHA50_RS20885 Protein ID WP_002892066.1
Coordinates 4034057..4034431 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GHA50_RS20875 4029209..4030402 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
GHA50_RS20880 4030425..4033571 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
GHA50_RS20885 4034057..4034431 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
GHA50_RS20890 4034458..4034676 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
GHA50_RS20895 4034835..4035401 + 567 WP_153459403.1 maltose O-acetyltransferase -
GHA50_RS27215 4035373..4035513 - 141 WP_004147370.1 hypothetical protein -
GHA50_RS20900 4035534..4036004 + 471 WP_153459404.1 YlaC family protein -
GHA50_RS20905 4035979..4037430 - 1452 WP_021313732.1 PLP-dependent aminotransferase family protein -
GHA50_RS20910 4037531..4038229 + 699 WP_002892021.1 GNAT family N-acetyltransferase -
GHA50_RS20915 4038226..4038366 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
GHA50_RS20920 4038366..4038629 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T142301 WP_002892050.1 NZ_CP045694:4034458-4034676 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T142301 NZ_CP060642:1608040-1608375 [Streptococcus pyogenes]
TTGGACTATAAGAAATATCAGATTATCTATGCTCCTGATGTTTTAGAGAAGCTAAAAGAAATTCGTGATTATATTTCTCA
AAACTACTCCTCAACATCAGGTCAGCGTAAAATGGAGCAAATCATTAGCGACATAGAAAAACTCGAGGTTTTCCCAGAAG
TTGGTTTTGATGCCGATGAAAAATATGGTTCAAAAATCAGCCATTATCACAGCACTAAAGGTTACACCTTAAGTAAAGAT
TATATTGTCCTATACCATATCGAGGGGGAAGAAAATAGGGTCGCTATTGATTACTTGCTTCCTACTCAAAGCGACTATAT
AAAGTTATTCAAATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT142301 WP_002892066.1 NZ_CP045694:4034057-4034431 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT142301 NZ_CP060642:1607763-1608050 [Streptococcus pyogenes]
ATGACTACAGTAAAAAAAAACAGAGCGGTTACCTTTCAAGCTAATAAAGAATTGGTAAGCGAAGCAATGACAGTATTAAA
CAAAAAAAATTTAACCTTATCATCTGCTTTAAGATTATTCCTACAAAATGTCGTTGTCACAAACGAGGTTGACTTATTGA
CGGAAGAGGAGTTAGAGAAAGAAAAACTTTTCAAGCAATTTCAAGCTGAAATCAACAAAAATATTGAGGACGTTCGTCAA
GGGAAGTTTTATACCTCTGAAGAAGTGAGGGCTGAACTTGGACTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References