Detailed information of TA system TA142289

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 737004..737782
• Replicon: chromosome
• Accession: NZ_CP045694
• Organism: Klebsiella pneumoniae strain TK421

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: GHA50_RS05275
• Protein ID: WP_031591140.1
• Coordinates: 737294..737782 (+)
• Length: 163 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: W8UEW1
• Locus tag: GHA50_RS05270
• Protein ID: WP_004150912.1
• Coordinates: 737004..737297 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GHA50_RS05250	732212..732814	-	603	WP_004174410.1	short chain dehydrogenase	-
GHA50_RS05255	732912..733823	+	912	WP_004181308.1	LysR family transcriptional regulator	-
GHA50_RS05260	733824..734972	-	1149	WP_153459139.1	PLP-dependent aspartate aminotransferase family protein	-
GHA50_RS05265	734983..736359	-	1377	WP_004217775.1	cystathionine beta-synthase	-
GHA50_RS05270	737004..737297	+	294	WP_004150912.1	DUF1778 domain-containing protein	Antitoxin
GHA50_RS05275	737294..737782	+	489	WP_031591140.1	GNAT family N-acetyltransferase	Toxin
GHA50_RS05280	737863..741605	-	3743	Protein_722	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (87-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (3-95)

Sequences

Toxin

Protein

• Length: 163 a.a.
• Molecular weight: 17604.53 Da
• Isoelectric Point: 7.7754

>T142289 WP_031591140.1 NZ_CP045694:737294-737782 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCGSDSNVLAYYSLASSAVMTNTAPGRFRRNMPDP
IPVVVLGRLAVDQSLHGRGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEAREFYLRVGFEPSPMDPMMLMVTLGDLV
HA

Nucleotide

• Length: 489 bp

>T142289 NZ_CP060593:98506-98985 [Rouxiella badensis]
ATGAGTTTACAATTGTCAGCTCCCGAGCCACTTACCACTGAACATATCCTTGATGACTTTGGTTGTGGGGAATCAACGTT
AGATGAATGGCTAAAACGGCGTGCTCTGACAAACCATTTAAACGGTGCAAGTCGTACTTTTGTTGTCGCGACTGACGATA
AAAGTGTTGTAGGTTATTATGCGCTTGCAGCCGGCGCTGTGACACACAGGGAAGCAACAGGGTCTGTTCGGCGCAATATG
CCTGATCCAGTTCCAGTGATAGTATTGGGAAGATTGGCGGTCGACCTTCGCACTCAGGGGCTAAAGCTCGGCGCGTCATT
GTTACAGGATGCCGTTCTTAGAGTTCGTTCAATTGCTGAAAACACAGGTGTCAGAGCATTGCTCGTACATGCACTTCATG
AACCGGCCAAACAATTTTATGAACACTATGGTTTTACGTCATCGCCAATTAACCCGATGACATTGATGTTACGCCTTTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10901.46 Da
• Isoelectric Point: 6.3783

>AT142289 WP_004150912.1 NZ_CP045694:737004-737297 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Nucleotide

• Length: 294 bp

>AT142289 NZ_CP060593:98249-98509 [Rouxiella badensis]
ATGCGTGATGCTGCGATCAATTTGCGTGCTTTGCCGGAACAACGTGATCTTATCGATCAAGCCGCCAGCCTATTAGGTAA
AAATCGATCTGATTTTATGTTGGAAGTAGCGTGCGAGCGTGCGCAGGCTATACTGCTCGATCAAGTATTTTTCCAGTTAG
ATTCTAAAAAATTTGATGAATTCACAGCGTTGCTTGATGCTCCTCAAAAGGCAAATCAGGGGCTGGAACGTCTGATGGAA
GTAAAAGCGCCTTGGGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GVL4