Detailed information of TA system TA142286

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 99036..99946
• Replicon: plasmid pTK421_2
• Accession: NZ_CP045692
• Organism: Klebsiella pneumoniae strain TK421

Toxin (Protein)

• Gene name: -
• Uniprot ID: R4YBC5
• Locus tag: GHA50_RS01330
• Protein ID: WP_004144067.1
• Coordinates: 99036..99506 (-)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: W9B1L8
• Locus tag: GHA50_RS01335
• Protein ID: WP_016528982.1
• Coordinates: 99503..99946 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GHA50_RS01295	95139..95836	+	698	Protein_107	IS1 family transposase	-
GHA50_RS01300	95836..96300	-	465	Protein_108	hypothetical protein	-
GHA50_RS01305	96369..96665	-	297	WP_094313394.1	hydrogenase expression/formation protein HypD	-
GHA50_RS01310	96899..97296	+	398	Protein_110	phosphonate ABC transporter substrate-binding protein	-
GHA50_RS01320	97784..97942	-	159	WP_115195635.1	type I toxin-antitoxin system Hok family toxin	-
GHA50_RS01325	98617..98925	+	309	WP_052463135.1	hypothetical protein	-
GHA50_RS01330	99036..99506	-	471	WP_004144067.1	RES family NAD+ phosphorylase	Toxin
GHA50_RS01335	99503..99946	-	444	WP_016528982.1	DUF2384 domain-containing protein	Antitoxin
GHA50_RS01340	100047..100554	-	508	Protein_115	DUF4113 domain-containing protein	-
GHA50_RS01345	100663..100884	-	222	WP_016528984.1	DUF2188 domain-containing protein	-
GHA50_RS01350	101029..101184	-	156	WP_016528985.1	hypothetical protein	-
GHA50_RS01355	101259..101636	-	378	Protein_118	TonB-dependent receptor	-
GHA50_RS01360	101633..101995	-	363	Protein_119	RNA polymerase subunit sigma	-
GHA50_RS01375	103870..104064	+	195	Protein_121	IS66 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	iucA / iucB / iucC / iucD / iutA / basG / clbI / iroB / iroC / iroD / iroN / rmpA	1..127353	127353

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (94-144)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17573.83 Da
• Isoelectric Point: 4.5152

>T142286 WP_004144067.1 NZ_CP045692:c99506-99036 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T142286 NZ_CP060592:c2782876-2782673 [Rouxiella badensis]
ATGAACAAAATCGACTATTTGATGCGTTTAAGAAAATGCACCACTATTGATACACTTGAACGTGTTATTGAGAAGAATAA
GTACGAACTCTCTAATGATGAGTTGGAGCTGTTTTACTCTGCGGCCGATCACCGTCTCGCCGAGCTGACGATGAATAAGC
TGTACGATAAAATCCCAACCTCCGTCTGGAAATTCGTACGCTAA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16494.83 Da
• Isoelectric Point: 10.2498

>AT142286 WP_016528982.1 NZ_CP045692:c99946-99503 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEIIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT142286 NZ_CP060592:c2783289-2782921 [Rouxiella badensis]
ATGGATGAGTACTCGCCTAAGCGACATGATATTGCGCAACTGCGGTTCCTCAACGAAAGTTTGTACGACGAAGGAATTGC
AACCCTGGGAGACAGCCATCACGGCTGGGTCAATGATCCAACTTCAGCAGTCAATCTGCAGTTGAATGACCTGATTGAGC
ATATTGCTTCTTTCGTCATGAGCTTTAAAATAAAATACCCGGATGAAACAAACCTCAGCGACCTGGTGGAAGAGTATCTC
GACGATACCTACACCTTGTTCAGCAATTATGGAATAAACGATTCTGATTTACGTCAATGGCAAAAAACCAAGAAACGACT
ATTCAGAATGTTCTCAGGGGATTATGTCTGTACCCTGATGAAAACATAA

Structures

Toxin

• AlphaFold DB: A0A384IED8

Antitoxin

• AlphaFold DB: W9B1L8