Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 51023..51548 Replicon plasmid pTK421_1
Accession NZ_CP045691
Organism Klebsiella pneumoniae strain TK421

Toxin (Protein)


Gene name ccdB Uniprot ID A0A1D8K3Q5
Locus tag GHA50_RS00305 Protein ID WP_006788214.1
Coordinates 51243..51548 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A379YN57
Locus tag GHA50_RS00300 Protein ID WP_063445614.1
Coordinates 51023..51241 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GHA50_RS00275 47222..47965 - 744 WP_153459027.1 hypothetical protein -
GHA50_RS00280 47997..48413 - 417 WP_153459028.1 type II toxin-antitoxin system VapC family toxin -
GHA50_RS00285 48410..48640 - 231 WP_153459029.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
GHA50_RS00290 48950..49399 + 450 WP_153459030.1 hypothetical protein -
GHA50_RS00295 49472..50464 - 993 WP_153459031.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
GHA50_RS26725 50608..50808 + 201 Protein_59 hypothetical protein -
GHA50_RS00300 51023..51241 + 219 WP_063445614.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
GHA50_RS00305 51243..51548 + 306 WP_006788214.1 type II toxin-antitoxin system toxin CcdB Toxin
GHA50_RS00310 51740..52756 + 1017 WP_065900162.1 hypothetical protein -
GHA50_RS00315 52954..53739 + 786 WP_063445612.1 site-specific integrase -
GHA50_RS00320 54606..55361 + 756 WP_063445611.1 replication initiation protein RepE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..130329 130329


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11687.46 Da        Isoelectric Point: 7.2015

>T142285 WP_006788214.1 NZ_CP045691:51243-51548 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRLMTTDMASVPASVTGEEV
ADLRHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T142285 NZ_CP060592:2281123-2281398 [Rouxiella badensis]
ATGGAAATACAGTGGACCCAAAAGGCCCAAGATGATCTGGAGCGTATTTATAGGTTTGCCTGCCAGTACAGTCGGCAACA
TGCTGACAACGTATTAGAACGTTTGATCATCGGAACCTCAGGGCTCGCCGACCATCCAAGAATCGGTGTACCTCAGACAA
GATATGAACCACGAGAAGTGAGGAAAATCTTGTTTGATGATTATGACGTCCACTATGAAATTCAGCGCGATACTATTTAT
ATTGTTGACCTGTGGCATACCCGAGAGGATCGCTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8307.23 Da        Isoelectric Point: 4.5203

>AT142285 WP_063445614.1 NZ_CP045691:51023-51241 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISGLVNTAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT142285 NZ_CP060592:2280871-2281119 [Rouxiella badensis]
ATGAGACAACTCGCAAAACAGACGATCAGCGCCCAGATCCCTGCAGAGCTTGCAGCTGCGGTTGAAAACTTGGCTATTGA
ACTGGATAGATCCAAAAGCTGGGTGATTAAAGAAGCGCTGACCGCCATGATTGAAGAGAGAGAGCGGCGGCATCAGCAGA
TTTTGAAAGGGCTTGCTGATGTCGATGCGGGAAGGGTGGTTAGCCATGCGGATGTAGTTGACTTCGCTAACAAACTGAAA
AAATCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1D8K3Q5

Antitoxin

Source ID Structure
AlphaFold DB A0A379YN57

References