Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 47997..48640 Replicon plasmid pTK421_1
Accession NZ_CP045691
Organism Klebsiella pneumoniae strain TK421

Toxin (Protein)


Gene name vagD Uniprot ID -
Locus tag GHA50_RS00280 Protein ID WP_153459028.1
Coordinates 47997..48413 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID -
Locus tag GHA50_RS00285 Protein ID WP_153459029.1
Coordinates 48410..48640 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GHA50_RS00265 43652..45877 - 2226 WP_032650494.1 exclusion suppressor FxsA -
GHA50_RS00270 45879..46967 - 1089 WP_114268690.1 transcriptional repressor PifC -
GHA50_RS00275 47222..47965 - 744 WP_153459027.1 hypothetical protein -
GHA50_RS00280 47997..48413 - 417 WP_153459028.1 type II toxin-antitoxin system VapC family toxin Toxin
GHA50_RS00285 48410..48640 - 231 WP_153459029.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
GHA50_RS00290 48950..49399 + 450 WP_153459030.1 hypothetical protein -
GHA50_RS00295 49472..50464 - 993 WP_153459031.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
GHA50_RS26725 50608..50808 + 201 Protein_59 hypothetical protein -
GHA50_RS00300 51023..51241 + 219 WP_063445614.1 type II toxin-antitoxin system antitoxin CcdA -
GHA50_RS00305 51243..51548 + 306 WP_006788214.1 type II toxin-antitoxin system toxin CcdB -
GHA50_RS00310 51740..52756 + 1017 WP_065900162.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..130329 130329


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-50)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15172.61 Da        Isoelectric Point: 8.5198

>T142284 WP_153459028.1 NZ_CP045691:c48413-47997 [Klebsiella pneumoniae]
VNKTYMLDTNICSFIMREQPEAVLKRLEQAVLRNQRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLKLEDWVK

Download         Length: 417 bp

>T142284 NZ_CP060592:2279507-2279827 [Rouxiella badensis]
ATGCAAATCTTATCTGTACCGGCCACGGTGCCGGTTGCGTCACGTCTGTCGCCCGTACAGGTCTGGCAGCAATTGTTAAC
GTATCTACTGGAGCACCATTACGGCCTGACGCTCAACGACACACCATTCCATGATGATGCGACCATTGAGGAGCATATCG
ACGCGGGAATAACGCTTGCCGATGCTGTTAATTTCCTGGTGGAACGCTATGAACTGGTGCGAACTGATCGTAAGGGTTTC
ACCTGGCAGGAGCAGACGCCATTCCTGACTGTCACCGATATTCTCAGAGCAAAGCGTGCTACCGGGCTGATGAATACGTA
A

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8736.84 Da        Isoelectric Point: 4.4692

>AT142284 WP_153459029.1 NZ_CP045691:c48640-48410 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIIREGDSIILRPVRPTWGSFAQLEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT142284 NZ_CP060592:2279105-2279440 [Rouxiella badensis]
ATGAATAAAGACGCTACCCAGACCTGGGGTCTTAAACGAGATATCACACCGTGCTTCGGAGCCCGGCTGGTGCAGGATGG
TCATCGTCTGAATTTTCTGGCAGACCGGGCAGGATTTACTGGTTCGTTCAGCGAGATGCAGGCCCGACAACTGGATGAAG
CCTTCCCGCATTTTGTCGCGCATATGGAACTGATGCTGCTCTCCGGTGAGCTGAATCCCCGGTGCGCGCACTGCGTCACA
CTGTACCGTAACGAGCTGACCTGTGAAGCCGATACCCTGGGCAGTCATGGGTATGTGTATATCGCCATTTATCCCTCAAA
TAAGCTTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References