Detailed information of TA system TA142283

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 37738..38381
• Replicon: plasmid pTK421_1
• Accession: NZ_CP045691
• Organism: Klebsiella pneumoniae strain TK421

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: GHA50_RS00220
• Protein ID: WP_153459023.1
• Coordinates: 37738..38154 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: GHA50_RS00225
• Protein ID: WP_153459024.1
• Coordinates: 38151..38381 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GHA50_RS00195	33428..34300	+	873	WP_153459019.1	alpha/beta fold hydrolase	-
GHA50_RS00200	34305..34673	+	369	WP_153459020.1	hypothetical protein	-
GHA50_RS00205	34670..35542	+	873	WP_153459021.1	DsbA family protein	-
GHA50_RS00210	35746..36726	+	981	WP_153459022.1	nitronate monooxygenase	-
GHA50_RS00215	36956..37609	-	654	WP_032733156.1	LysE family translocator	-
GHA50_RS00220	37738..38154	-	417	WP_153459023.1	type II toxin-antitoxin system VapC family toxin	Toxin
GHA50_RS00225	38151..38381	-	231	WP_153459024.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
GHA50_RS00230	38827..39207	+	381	WP_015874975.1	YrzE family protein	-
GHA50_RS00235	39364..40104	-	741	WP_009653143.1	abortive infection family protein	-
GHA50_RS00240	40140..40928	-	789	WP_117054846.1	DUF2971 domain-containing protein	-
GHA50_RS00245	40925..41545	-	621	WP_117054845.1	hypothetical protein	-
GHA50_RS00250	41542..42237	-	696	WP_153459025.1	DNA-binding protein	-
GHA50_RS00255	42423..42713	+	291	WP_153459026.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..130329	130329

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-44)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15086.47 Da
• Isoelectric Point: 6.4534

>T142283 WP_153459023.1 NZ_CP045691:c38154-37738 [Klebsiella pneumoniae]
VNKIYMLDTNICSFIMREQPEAVLKRLEQAVLRNQRIVISAITYSEMCFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVN

Nucleotide

• Length: 417 bp

>T142283 NZ_CP060592:c979286-978819 [Rouxiella badensis]
GTGGCCCTGTGGCAATGTGATTTAAGGGTGTCATGGCGCAGTCAGCTGTTTTCGCTGGCGGTGCATGGCATTCTTATTCT
GCTGATTCTTCTGGCTCCCTGGCCGCCCGACGACTGGATGATTTGGCTGATACTGCTAATTATTGTGGTCTCTCAGTCAA
TACGCAGCCAGCGGCGCATCAGTGCGCGCCAGGGTGAAATCAGCCTTCTTGCTCAGGACAGACTGCTGTGGCGTAAACAG
GAATGGAAAATTAAGCAGCCGGTCTGGATGATAGACAGCGGTATACTGCTCTCGCTGAGGCGTGAACGCACCGGCCATGG
CTGGCGTCAGGCGCTGCGCGGCAAGAAACACAAACTTTGGCTGGCTTCTGACAGCATGAGTCAGGAGGAGTGGCGGCATC
TGCGGCAGATATTATTGACCGGCAAGCGCGACGCGGCACCGGCCAATAATAATCACCACCCGCTGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8760.91 Da
• Isoelectric Point: 4.6286

>AT142283 WP_153459024.1 NZ_CP045691:c38381-38151 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIIREGDSIILRPVRPTWGSFVHLEKADPDFMAERESVVTDEGRFDL

Nucleotide

• Length: 231 bp

>AT142283 NZ_CP060592:c979533-979267 [Rouxiella badensis]
ATGGATATAAACAACAAACCCCGCATCCACTGGGCCTGTCGTCGCGGCATGAAAGAGCTGGACCTTGCCATCCTGCCGTT
CTTTGAATATGAGTTCGACACCCTAAGTGATGAAGACAAGCAGCAGTTCGCCCGACTGCTTGAATGTGACGACCCAGACC
TTTTTAACTGGCTGATGAACTACGGCGAGCCGAACGATCTCGAGTTGAAGCGCATGGTTAACCTGATTCAGACACGAAAC
AAACAACGTGGCCCTGTGGCAATGTGA