Detailed information of TA system TA142278

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 104029..104699
• Replicon: plasmid pWSD411_2
• Accession: NZ_CP045675
• Organism: Klebsiella pneumoniae strain WSD411

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: F8D37_RS25840
• Protein ID: WP_004213072.1
• Coordinates: 104029..104472 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: F8D37_RS25845
• Protein ID: WP_004213073.1
• Coordinates: 104469..104699 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F8D37_RS25805	99438..99713	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
F8D37_RS25810	99776..100267	+	492	WP_011251327.1	DM13 domain-containing protein	-
F8D37_RS25815	100316..101236	+	921	WP_029884592.1	DUF1471 domain-containing protein	-
F8D37_RS25820	101327..101733	+	407	Protein_102	GAF domain-containing protein	-
F8D37_RS29010	102248..102886	-	639	WP_032488580.1	mucoid phenotype regulator RmpA2	-
F8D37_RS25830	103302..103595	+	294	Protein_104	transposase	-
F8D37_RS25835	103599..104015	+	417	Protein_105	restriction endonuclease	-
F8D37_RS25840	104029..104472	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
F8D37_RS25845	104469..104699	-	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
F8D37_RS25850	105307..106440	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
F8D37_RS25855	106456..106749	+	294	WP_004213076.1	hypothetical protein	-
F8D37_RS25860	106739..106945	-	207	WP_004213077.1	hypothetical protein	-
F8D37_RS25865	107297..107587	+	291	WP_004213078.1	hypothetical protein	-
F8D37_RS25870	107577..108476	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iucA / iucB / iucC / iucD / iutA / rmpA	1..177848	177848

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T142278 WP_004213072.1 NZ_CP045675:c104472-104029 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T142278 NZ_CP060584:948734-948886 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACGGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATCTCTGATCAAAATGATCAAGAAAAGAATTCTGAAGAAGACAGTCAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT142278 WP_004213073.1 NZ_CP045675:c104699-104469 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT142278 NZ_CP060584:948110-948709 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTTCTAGATGAATTAAAGAAAAATGTTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACAGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620