Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 58330..59066 Replicon plasmid pWSD411_2
Accession NZ_CP045675
Organism Klebsiella pneumoniae strain WSD411

Toxin (Protein)


Gene name tacT Uniprot ID A0A2J5Q928
Locus tag F8D37_RS25590 Protein ID WP_004098919.1
Coordinates 58584..59066 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A7D3T1D0
Locus tag F8D37_RS25585 Protein ID WP_004213599.1
Coordinates 58330..58596 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F8D37_RS25565 55843..56847 + 1005 WP_000427623.1 IS110-like element IS4321 family transposase -
F8D37_RS25570 57133..57627 + 495 WP_004213594.1 hypothetical protein -
F8D37_RS25575 57688..57891 + 204 WP_004213596.1 hemolysin expression modulator Hha -
F8D37_RS25580 57905..58135 + 231 WP_004213598.1 hypothetical protein -
F8D37_RS25585 58330..58596 + 267 WP_004213599.1 DUF1778 domain-containing protein Antitoxin
F8D37_RS25590 58584..59066 + 483 WP_004098919.1 GNAT family N-acetyltransferase Toxin
F8D37_RS25595 59267..60670 + 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
F8D37_RS25600 60699..61331 - 633 WP_001567369.1 hypothetical protein -
F8D37_RS25605 61770..62997 + 1228 Protein_59 IS3 family transposase -
F8D37_RS25610 62993..63388 - 396 Protein_60 DDE-type integrase/transposase/recombinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - iucA / iucB / iucC / iucD / iutA / rmpA 1..177848 177848
- inside IScluster/Tn - - 47012..62997 15985


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(66-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17335.06 Da        Isoelectric Point: 10.0704

>T142277 WP_004098919.1 NZ_CP045675:58584-59066 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T142277 NZ_CP060584:829868-830185 [Staphylococcus aureus]
ATGAATTGGGAAATTAAAGATTTGATGTGTGATATTGAAGTGATAAAAGAAAAAATCAATGATGTAGCTATCAAACATGG
TTGGTTTGTTGAAGATAAATTTGTCAAAAATGAATTAGAAACAAAACAGGAACATATTAATTTTTCTGCTAGCTATTTAG
AACATCGTATACAAAATGAACATACAGTTGAGTTATTACAGGTGTACTTAAAAGAATTCGGTGAACTTATACAAAAGTTT
CATGAAATAGAAAAAGCATCATCTGAGAACTTTGGCGAGGAATCAGATGACGCAAAGAAATTAAAAATTACAGAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10151.58 Da        Isoelectric Point: 4.5141

>AT142277 WP_004213599.1 NZ_CP045675:58330-58596 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRVLIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT142277 NZ_CP060584:829656-829865 [Staphylococcus aureus]
ATGAATAATGAACAAAAAGAAGTAATAGAACACGTGGTTTATCAACTTGAGTTAAGTATCATGAATAATTTTGAAAGTTA
TGAACACACGGAATATGTTGATGGTATTGAAGTGGTTTCAGAGATCAGTCCTGAAAAGCACTTAGAATTGATAATGAAAT
GGTGCGCACAAGAATTAAAGAATAATTTTCAATTAGAAAAAGGAGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J5Q928

Antitoxin

Source ID Structure
AlphaFold DB A0A7D3T1D0

References