Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5287084..5287709 Replicon chromosome
Accession NZ_CP045661
Organism Klebsiella pneumoniae strain SMU18037509

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag GFJ44_RS25290 Protein ID WP_002882817.1
Coordinates 5287084..5287467 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag GFJ44_RS25295 Protein ID WP_004150355.1
Coordinates 5287467..5287709 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GFJ44_RS25275 5284450..5285352 + 903 WP_004901686.1 formate dehydrogenase subunit beta -
GFJ44_RS25280 5285349..5285984 + 636 WP_004901683.1 formate dehydrogenase cytochrome b556 subunit -
GFJ44_RS25285 5285981..5286910 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
GFJ44_RS25290 5287084..5287467 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
GFJ44_RS25295 5287467..5287709 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
GFJ44_RS25300 5287914..5288831 + 918 WP_004901674.1 alpha/beta hydrolase -
GFJ44_RS25305 5288845..5289786 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
GFJ44_RS25310 5289831..5290268 - 438 WP_153517936.1 D-tyrosyl-tRNA(Tyr) deacylase -
GFJ44_RS25315 5290265..5291125 - 861 WP_002882807.1 virulence factor BrkB family protein -
GFJ44_RS25320 5291119..5291718 - 600 WP_004901669.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T142273 WP_002882817.1 NZ_CP045661:c5287467-5287084 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T142273 NZ_CP060584:432736-433002 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT142273 WP_004150355.1 NZ_CP045661:c5287709-5287467 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT142273 NZ_CP060584:432485-432736 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References