Detailed information of TA system TA142272

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4961704..4963385
• Replicon: chromosome
• Accession: NZ_CP045661
• Organism: Klebsiella pneumoniae strain SMU18037509

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: J2XCI1
• Locus tag: GFJ44_RS23780
• Protein ID: WP_004146663.1
• Coordinates: 4961704..4962621 (-)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: A6TH05
• Locus tag: GFJ44_RS23785
• Protein ID: WP_004177809.1
• Coordinates: 4962627..4963385 (-)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GFJ44_RS23760	4957010..4958530	+	1521	WP_002885173.1	sugar ABC transporter ATP-binding protein	-
GFJ44_RS23765	4958527..4959525	+	999	WP_002885172.1	ribose ABC transporter permease	-
GFJ44_RS23770	4959556..4960497	+	942	WP_002885156.1	ABC transporter substrate-binding protein	-
GFJ44_RS23775	4960572..4961579	+	1008	WP_004221949.1	carbohydrate kinase family protein	-
GFJ44_RS23780	4961704..4962621	-	918	WP_004146663.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
GFJ44_RS23785	4962627..4963385	-	759	WP_004177809.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
GFJ44_RS23790	4963471..4964199	-	729	WP_004177810.1	response regulator transcription factor	-
GFJ44_RS23795	4964472..4966619	+	2148	WP_012737173.1	formate dehydrogenase H subunit alpha, selenocysteine-containing	-
GFJ44_RS23800	4966965..4967651	+	687	WP_032421940.1	sel1 repeat family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34659.10 Da
• Isoelectric Point: 5.4707

>T142272 WP_004146663.1 NZ_CP045661:c4962621-4961704 [Klebsiella pneumoniae]
MDIQAPYYRQVALLMQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRAIALPHIRDALARIAANLQQQ
AGMSAVLQANRSDEMRVIVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQH
PRDFYDVKLLLDAQELDRPIFNGFIAYLLSHNRPLAEVLNPRWKDIAEPFYREFSGMTFETIALEELTAVPNRMIAALKS
CFTQQDVDFLLSFKRGEPDWRLAPEMRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 918 bp

>T142272 NZ_CP060584:407716-407823 [Staphylococcus aureus]
ATGTTCAATTTATTAATTAACATCATGACTTCAGCTATAAGCGGCTGTCTTGTTGCGTTTTTTGCACATTGGTTACGAAC
GCGCAACAATAAAAAAGGTGACAAATAA

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28548.92 Da
• Isoelectric Point: 10.0870

>AT142272 WP_004177809.1 NZ_CP045661:c4963385-4962627 [Klebsiella pneumoniae]
MATKLNWLLQNSAPGDVILQSWLSQHAISPSLAFKYAQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSVENKALLPKWFREFPGVEWIVISGKKLPVLDDRYRVTLDIKGKKLIGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQAKRLYLFFASFYEHGWLKRIDSQKIDLGAGKRQIVENGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

>AT142272 NZ_CP060584:c407900-407840 [Staphylococcus aureus]
TACATAATAAATTGAACATCTAAATACACCAAATCCCCTCACTACTGCCATAGTGAGGGGA

Structures

Toxin

• AlphaFold DB: J2XCI1

Antitoxin

• AlphaFold DB: A6TH05