Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4671469..4672279 Replicon chromosome
Accession NZ_CP045661
Organism Klebsiella pneumoniae strain SMU18037509

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag GFJ44_RS22430 Protein ID WP_004178461.1
Coordinates 4671469..4672002 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID A0A483I2M4
Locus tag GFJ44_RS22435 Protein ID WP_004222378.1
Coordinates 4672013..4672279 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GFJ44_RS22425 4670300..4671421 + 1122 WP_002887282.1 cupin domain-containing protein -
GFJ44_RS22430 4671469..4672002 - 534 WP_004178461.1 GNAT family N-acetyltransferase Toxin
GFJ44_RS22435 4672013..4672279 - 267 WP_004222378.1 DUF1778 domain-containing protein Antitoxin
GFJ44_RS22440 4672382..4673815 - 1434 WP_004222374.1 Cu(+)/Ag(+) sensor histidine kinase -
GFJ44_RS22445 4673805..4674488 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
GFJ44_RS22450 4674660..4676045 + 1386 WP_004222371.1 efflux transporter outer membrane subunit -
GFJ44_RS22455 4676063..4676407 + 345 WP_004222368.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T142270 WP_004178461.1 NZ_CP045661:c4672002-4671469 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T142270 NZ_CP060528:1885051-1885512 [Staphylococcus epidermidis]
ATGGGGTTATATGAAGAAATTTGTATAGATAATGATATGGTAGAAATTAAAGAAACTAACAAACTACCATCTTTCCAATC
GGGATTTTATATGAACGGAACTGTTTATATAAAAAGTGACTTGTCCGAAAAAGAAAAAATAAAAGTTCTCTACGAAGAAC
TGGCACATCATAAACTTACATATGGAAACATCTTAGACCAATCTAAATTCAACAACCGTAAATTTGAAAACTACGCTAGG
CGTCACGGATATGAAGCTGCTCTACCTTTGCGCATTATTGTGGAGGCGCACAACTATGGTGTTAGTAACTTATATGAATT
AGCTGAATATGTTCAATTAAGTGAAGAGTACATAGCAGAAATATTGAAACATTACAAAAATAAATATGGTATTGGAACTC
ACTACGAAGAATACCTAATTACATTTGATCCGTTGAGAGTTTTTAAATATAAAGAAATATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9969.44 Da        Isoelectric Point: 10.1757

>AT142270 WP_004222378.1 NZ_CP045661:c4672279-4672013 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLSEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT142270 NZ_CP060528:1884710-1885039 [Staphylococcus epidermidis]
ATGAGAACAAATGATGAAATAATCACAATAATTAAAACAACATTAAAAGAACAAAACATGTCACTTAGTGAATTAGCTCG
TCGTGTAGGAATGGCCAAATCTGCTGTATCACGTTACTTGAATTTAACAAGAGAATTTCCGTTAAACCGTGCGGAAGATT
TTGCGAACGCACTCCATATTAGTACAGAGTATTTACTTGGATTTAATGGAAGTGAACAAACAAGACAACAAGACACTATG
GCTGCTCATTTTGACAAAGAGGGTCTAACAGAAGAAGAAATCGAAGAAGTCAATAAGTTTATTGAGTGGGTTAAGAATAG
AGACAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
AlphaFold DB A0A483I2M4

References