Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/RelB(antitoxin)
Location 1078694..1079330 Replicon chromosome
Accession NZ_CP045646
Organism Bifidobacterium breve strain JSRL01

Toxin (Protein)


Gene name mazF Uniprot ID D4BP71
Locus tag GFJ40_RS04535 Protein ID WP_003829234.1
Coordinates 1078694..1079050 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID D6PAX1
Locus tag GFJ40_RS04540 Protein ID WP_012577921.1
Coordinates 1079037..1079330 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GFJ40_RS04505 1073886..1074797 + 912 WP_003829224.1 ABC transporter ATP-binding protein -
GFJ40_RS04510 1074994..1075620 + 627 WP_003829226.1 30S ribosomal protein S4 -
GFJ40_RS04515 1075957..1076739 - 783 WP_042994102.1 gamma-glutamyl-gamma-aminobutyrate hydrolase family protein -
GFJ40_RS04520 1076879..1077364 - 486 WP_003829229.1 cytidine deaminase -
GFJ40_RS04525 1077454..1077810 + 357 WP_042994100.1 SdpI family protein -
GFJ40_RS04530 1077915..1078517 + 603 WP_003829233.1 transglutaminase family protein -
GFJ40_RS04535 1078694..1079050 - 357 WP_003829234.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
GFJ40_RS04540 1079037..1079330 - 294 WP_012577921.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
GFJ40_RS04550 1079745..1080419 + 675 WP_015438748.1 histidine phosphatase family protein -
GFJ40_RS04555 1080502..1080906 + 405 WP_014483805.1 DUF948 domain-containing protein -
GFJ40_RS04560 1080906..1081142 + 237 WP_003829239.1 hypothetical protein -
GFJ40_RS04565 1081238..1083916 + 2679 WP_014483804.1 alanine--tRNA ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-110)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(11-53)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 13316.23 Da        Isoelectric Point: 7.9984

>T142251 WP_003829234.1 NZ_CP045646:c1079050-1078694 [Bifidobacterium breve]
MMKTDPHQFEIWWVPFTFPDKPGKAKNRPSVILQWDDGTRIALVTKVTGNTWRDEPGYVVLRDWQDAGLSKPSAVRCSQL
LRLPSSLFLDDGPTGRLSAYDAGRVTWAINELYPGLLA

Download         Length: 357 bp

>T142251 NZ_CP060494:c2835901-2835632 [Sphingosinithalassobacter sp. CS137]
GTGAAGCTCATCTGGCGCCCGGAGGCGCGCCGGAGCCTGCTCGACATCACCGACTATATCGCCGAGCGCAATCCATCGGC
GGCCCGGCGGCTCCGCGACGCCATCCTGCGCACCGCAGACCGGCTCCCCGATCATCCGTATCTCCACCGCCCGGGCCGCG
TCCCCGGCACGCGCGAAGCGGTGATCCACCCCACCACCATCCTCGTGGATCGCGTCGGCGAAAGCATCGAGATTCTCGCC
GTCCTCCACACCCGCCGGGAGTATCCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10866.31 Da        Isoelectric Point: 4.2896

>AT142251 WP_012577921.1 NZ_CP045646:c1079330-1079037 [Bifidobacterium breve]
MAIKEKTTISLDAQTKRDGIAILDAMGLNLSTFAEMSLRQLVRDGRLPFTPSVRPSFEKDNEGYPLFKANMDDPRIVTPQ
IRDGAVILPEGWDDDED

Download         Length: 294 bp

>AT142251 NZ_CP060494:c2836080-2835898 [Sphingosinithalassobacter sp. CS137]
ATGGGCAAGCTCGATCCAATCGTGTCCGAATTCGACACCGACGAGGAAGCCGAGGCGTATGACGCCTGGTTCCGCGCCAA
GGTGGAGGCCGCGATGGCGTCTCCCGGCCCCGGCATTCCGCACGATCAGGTGATGGCCGAAATGCAGGCGATCATCGACA
AGCATCGGCAAACGGCGAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0L7B6G7

Antitoxin

Source ID Structure
AlphaFold DB A0A1S2VTZ6

References