Detailed information of TA system TA142250

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-RelB
• Location: 1018849..1019404
• Replicon: chromosome
• Accession: NZ_CP045646
• Organism: Bifidobacterium breve strain JSRL01

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: S2ZXD7
• Locus tag: GFJ40_RS04250
• Protein ID: WP_003829109.1
• Coordinates: 1018849..1019172 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: S2ZDC4
• Locus tag: GFJ40_RS04255
• Protein ID: WP_003829112.1
• Coordinates: 1019159..1019404 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GFJ40_RS04225	1013876..1014174	-	299	Protein_814	IS256 family transposase	-
GFJ40_RS04230	1014645..1015910	-	1266	WP_014483848.1	Fic family protein	-
GFJ40_RS04235	1016452..1016817	-	366	WP_013140585.1	YccF domain-containing protein	-
GFJ40_RS04240	1017055..1017924	+	870	WP_003829104.1	class I SAM-dependent methyltransferase	-
GFJ40_RS04245	1018018..1018632	+	615	WP_014483846.1	cupin domain-containing protein	-
GFJ40_RS04250	1018849..1019172	-	324	WP_003829109.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
GFJ40_RS04255	1019159..1019404	-	246	WP_003829112.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
GFJ40_RS04260	1019578..1020834	+	1257	WP_016463167.1	HipA domain-containing protein	-
GFJ40_RS04265	1021099..1021290	+	192	WP_003829118.1	hypothetical protein	-
GFJ40_RS04270	1021474..1022745	-	1272	WP_042994115.1	MFS transporter	-
GFJ40_RS04275	1022902..1023564	-	663	WP_042994114.1	TetR/AcrR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1008618..1044034	35416

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-103)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (5-56)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11859.67 Da
• Isoelectric Point: 5.0771

>T142250 WP_003829109.1 NZ_CP045646:c1019172-1018849 [Bifidobacterium breve]
MKRGEIWTLQADGYASKPRPVVIVQSDAVDRFDSVITCLLTSYDSSDIDTRVRLEPSPENGLNKVSYVMTDKIVTVDRKL
LGYQVGVVDDAAMANIGRQLMRVLGLL

Nucleotide

• Length: 324 bp

>T142250 NZ_CP060493:840360-840851 [Bifidobacterium longum]
ATGACCGACGTTTCCATGCAGCGTATTTTGCTTGATACCAATGTACTGTTGGACTATTTGCTCCATCGCGATGATCACGC
CAAGATGGCGGAATCGGTTATGGAACTTGGCGCGAAGAACAACGTTACGTTGTTGTGCGCGTCATTGTCGCTGAAGGATA
TCGCATACCTATCATCATCGGCGATTCGTAGGGAATTCAAATCGAATGAATCGGAAGTGGAGAATTTCACTCGAAGCTTT
CTGTCGTCACGGGTGCCATGGCATTGTATCGAGCAGGTGAAGGAGATGTGTGACATCGTCGCTGTCGATGAGTCGACATG
TGATAAGGCTTTTTCCCTGCAAAAACGGCATCGGGATTTTGAAGATGATCTGATCATCGTTGCCGCGCAACAATCCGGTG
CCAACTGTGTGGTCACATCGGATGCCGAATTAATCAGTCATTTTCCCGAATATTGCAAAACACCGGCTGAAATCGTGGCG
GCGCTGGAATAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9339.43 Da
• Isoelectric Point: 4.5881

>AT142250 WP_003829112.1 NZ_CP045646:c1019404-1019159 [Bifidobacterium breve]
MSMQIATRVDDEQAALFKETTRQLGTTPADALRMFISAFNDYRGFPYEVRLPRNDVEPFASERDATEYASRLALRMSDET
R

Nucleotide

• Length: 246 bp

>AT142250 NZ_CP060493:839998-840360 [Bifidobacterium longum]
ATGACCATGGTCCAAATGAACGTTCGCATTGATGCGAAACTGAAGAACGAGGTCGAAGAAGTCCTCAAGGGCAAAGGCGT
ATCGGTGAGTGACGTAATCCGGTCGCTGTGGTTGTACATCGCCGACCGCAAGGAAGTGCCGGCATTGGAAACGACCGCCG
AAGAGCAGGCCCGCGAGGCGGAAAAGCAGCGCAAGCTGAAACTGATTCGAGAAGGTGCCGGATACGTGCACAAGGAACTG
GTCAAGGCCGGACTGATCTCAGAAGACACCGACCTCATGGAAGGCCTTACCTACAAGCAGTTCCGCGACAAAATGTACGA
CGAGCAACTTGATGCGTATTACAACATGAAGAGGGTCGATTGA

Structures

Toxin

• AlphaFold DB: A0A087B6Q7

Antitoxin

• AlphaFold DB: A0A087B6Q6