Detailed information of TA system TA142232

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 316169..316755
• Replicon: chromosome
• Accession: NZ_CP045641
• Organism: Klebsiella quasipneumoniae strain KP18-31

Toxin (Protein)

• Gene name: doc
• Uniprot ID: W8VD46
• Locus tag: GFK78_RS01440
• Protein ID: WP_002920800.1
• Coordinates: 316387..316755 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: GFK78_RS01435
• Protein ID: WP_048295677.1
• Coordinates: 316169..316390 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GFK78_RS01415	312326..313252	+	927	WP_012540389.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
GFK78_RS01420	313249..314526	+	1278	WP_008806971.1	branched chain amino acid ABC transporter permease LivM	-
GFK78_RS01425	314523..315290	+	768	WP_008806972.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
GFK78_RS01430	315292..316005	+	714	WP_004145133.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
GFK78_RS01435	316169..316390	+	222	WP_048295677.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
GFK78_RS01440	316387..316755	+	369	WP_002920800.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
GFK78_RS01445	317046..318362	+	1317	WP_004174008.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
GFK78_RS01450	318469..319356	+	888	WP_004202113.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
GFK78_RS01455	319353..320198	+	846	WP_004202115.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
GFK78_RS01460	320200..321270	+	1071	WP_017899977.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	313249..322007	8758

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (13-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13552.92 Da
• Isoelectric Point: 8.6410

>T142232 WP_002920800.1 NZ_CP045641:316387-316755 [Klebsiella quasipneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T142232 NZ_CP060470:2248074-2248364 [Xanthomonas citri pv. citri]
ATGCCACAAGTAATCTTTGCGCCGGCGGCGATCGGGGACATGCAACGGCTGCGCGAATTCCTGAAGCCGAAGAGTCCCGA
TGCCGCGAGGCGGGCCGGTGAAGCGATTAGGCGCGGCGTCCAGGCTCTCGGTGCCCACCCTCGAATGGGGCGGCTCATAG
AAGATCTGCCTGAACAGTATCGGGAGTGGCTTATCGATTTTGGGGATAGTGGCTATGTGGCTCGGTACCGCCACGCTGGC
GATACCGTCACGATTTTGGCCGTGCGACACCAGAAGGAAGCGGGGTACTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8135.20 Da
• Isoelectric Point: 5.0637

>AT142232 WP_048295677.1 NZ_CP045641:316169-316390 [Klebsiella quasipneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGVEIRELADK

Nucleotide

• Length: 222 bp

>AT142232 NZ_CP060470:2247808-2248086 [Xanthomonas citri pv. citri]
ATGGTTACGGCAACTTCCATCAAGCTTGATGATGAATTGAAAGGGCGGGTTCAGCACTTGGCCGAGGCGCGCCGGCGTAC
CCCGCATTGGATCATGCGCGAAGCCATTGAGCAGTACGTCGAGCGCGAGGAAAAGCGTGAGGCGTTGAACCGGGACACGC
TCAAGGCATGGGATGAATTTCAGGCGACGGGCCTGCACGTGACCGCTGAGGAAGTGGACAAGTGGCTCGCGAGCTGGGGA
ACAGACGACGAACTGCCTCCGCCAGAATGCCACAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0H3GUD1