Detailed information of TA system TA142213

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 82240..83717
• Replicon: plasmid unnamed2
• Accession: NZ_CP045633
• Organism: Roseibium aggregatum strain ZYF612

Toxin (Protein)

• Gene name: HipA1
• Uniprot ID: -
• Locus tag: GFK91_RS29275
• Protein ID: WP_229006407.1
• Coordinates: 82240..83496 (-)
• Length: 419 a.a.

Antitoxin (Protein)

• Gene name: HipB2
• Uniprot ID: -
• Locus tag: GFK91_RS29280
• Protein ID: WP_229006408.1
• Coordinates: 83493..83717 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GFK91_RS29250 (GFK91_29120)	77794..78828	+	1035	WP_229006402.1	protease modulator HflC	-
GFK91_RS29255 (GFK91_29125)	78825..79220	+	396	WP_229006403.1	hypothetical protein	-
GFK91_RS29260 (GFK91_29130)	79307..79525	-	219	WP_229006404.1	hypothetical protein	-
GFK91_RS29265 (GFK91_29135)	79584..79913	-	330	WP_229006405.1	hypothetical protein	-
GFK91_RS29270 (GFK91_29140)	80518..81903	+	1386	WP_229006406.1	transposase	-
GFK91_RS29275 (GFK91_29145)	82240..83496	-	1257	WP_229006407.1	type II toxin-antitoxin system HipA family toxin	Toxin
GFK91_RS29280 (GFK91_29150)	83493..83717	-	225	WP_229006408.1	helix-turn-helix domain-containing protein	Antitoxin
GFK91_RS29285 (GFK91_29155)	84034..84381	-	348	WP_229006409.1	hypothetical protein	-
GFK91_RS29290 (GFK91_29160)	84933..85799	+	867	WP_229006410.1	DUF932 domain-containing protein	-
GFK91_RS29295	86062..86967	-	906	WP_229006453.1	hypothetical protein	-
GFK91_RS29300 (GFK91_29170)	87657..88208	-	552	WP_229006411.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..254527	254527
	-	flank	IS/Tn	-	-	80518..81903	1385

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (5-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (11-65)

Sequences

Toxin

Protein

• Length: 419 a.a.
• Molecular weight: 46225.03 Da
• Isoelectric Point: 5.0416

>T142213 WP_229006407.1 NZ_CP045633:c83496-82240 [Roseibium aggregatum]
MSRALDVYLRDDKVGRLEQNDSGQLSFAYAPDYCTQNKVAISVSMPLCDEVYPDPVARPFFSGLLPDERARQRLASALGV
SRENAFGLLEIIGGECAGALSLVPEGWDLPAFDTCETEPLNEEQLVKVLDLLRQRPLLGGEEGVRLSLAGAQDKLAVCVQ
DGQISLPKNGRPTTHVLKPFIEGLEGTVENELFCMMLAARMGLDAPSVSKSSAGKTDFILVERYDRQVEKDGSIRKLHQE
DFCQALGVPPELKYEEEGGPGVSRSQELIQEWTQRPAADRLTFQRMLIFHYLVGNADAHAKNYALLYRDWTPDLAPIYDV
VCTAAYPRLAKKMAMQIGGRSLPDTILLEHWLTLVPASKSAQRLLTRELAKMTSEIGGKADALLEELGEAGTYHPILKTV
RRIIETRAKLVLSTIEKA

Nucleotide

• Length: 1257 bp

>T142213 NZ_CP060460:99875-100174 [Xanthomonas citri pv. citri]
ATGGCTGAAATAATCTGGTCGGTGCCGGCATTGGCTGATCTGGACGCAATTGCCGACTACATCGCAATCGATAACGCACC
CGCTGCAGCGGCGCTGGTGAAACGGGTGTTCGGGCACGTTGAGCAATTGATCGAACACCCGGACAGCGGAAGCCGACCAC
AGGAACTCAAGCGCTCGCGCTATCGCCAGATTGTCGAACCACCTTGCCGCGTGTTCTACCGCGTGGATGGGCAGCGCATC
GTGGTGGTGCACGTCATGCGGTCGGAGCGCGCGCTTCGCGGGAACCGTCTTTCACGCTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8182.48 Da
• Isoelectric Point: 10.9762

>AT142213 WP_229006408.1 NZ_CP045633:c83717-83493 [Roseibium aggregatum]
MNIIKDQHQLGQLIRAERKRQRLTQEQLAGLSGVGVRFVRELEHGKEGCHIGLAFTVMQTLGLSVQVTGRGSTV

Nucleotide

• Length: 225 bp

>AT142213 NZ_CP060460:99630-99887 [Xanthomonas citri pv. citri]
ATGCGCACCGAACTTGTCACCACGCTCAAGCGTCAGGCGACCGAACTCCTTGCAGCTGCAGAGCGCGACAAGGAGCCAAT
CCTGATCACACAGCACGGTTTACCCAGTGCCTATCTGGTGGATGTTGCCAGCTATGAGCGCATGCAGCAGCGCATTGCGC
TCCTCGAAGGAATCGCCCGTGGAGAAATGGCAGTCGCGGAAGGCCGTACGCTGAGCCATGAACAGGCACGGCACCGCATG
GCGCGATGGCTGAAATAA