Detailed information of TA system TA142207

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 60583..61613
• Replicon: plasmid unnamed2
• Accession: NZ_CP045624
• Organism: Roseibium aggregatum strain SDL044

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: GFK90_RS29320
• Protein ID: WP_007803220.1
• Coordinates: 60583..61152 (-)
• Length: 190 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: V9WPQ5
• Locus tag: GFK90_RS29325
• Protein ID: WP_007803221.1
• Coordinates: 61149..61613 (-)
• Length: 155 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GFK90_RS29295 (GFK90_29115)	56157..56681	-	525	Protein_65	IS110 family transposase	-
GFK90_RS29300 (GFK90_29120)	56741..57496	-	756	WP_228866800.1	TniQ family protein	-
GFK90_RS29305 (GFK90_29125)	57529..58407	-	879	WP_007803215.1	TniB family NTP-binding protein	-
GFK90_RS29310 (GFK90_29130)	58498..59430	-	933	WP_007803217.1	IS5-like element ISRhba5 family transposase	-
GFK90_RS29315 (GFK90_29135)	59682..60524	+	843	WP_007803218.1	hypothetical protein	-
GFK90_RS29320 (GFK90_29140)	60583..61152	-	570	WP_007803220.1	PIN domain-containing protein	Toxin
GFK90_RS29325 (GFK90_29145)	61149..61613	-	465	WP_007803221.1	excisionase family DNA-binding protein	Antitoxin
GFK90_RS29330 (GFK90_29150)	61763..62683	-	921	WP_228866870.1	helix-turn-helix domain-containing protein	-
GFK90_RS29335 (GFK90_29155)	62844..64478	+	1635	WP_228866801.1	Mu transposase C-terminal domain-containing protein	-
GFK90_RS29340 (GFK90_29160)	64480..65361	+	882	WP_228866802.1	TniB family NTP-binding protein	-
GFK90_RS29345 (GFK90_29165)	65358..66392	+	1035	WP_228866803.1	TniQ family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..96518	96518
	-	flank	IS/Tn	-	-	58498..59430	932

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-113)

Antitoxin

• SinI-like, DNA-binding domain (81-127)

Sequences

Toxin

Protein

• Length: 190 a.a.
• Molecular weight: 21163.39 Da
• Isoelectric Point: 5.2804

>T142207 WP_007803220.1 NZ_CP045624:c61152-60583 [Roseibium aggregatum]
MSQYTVLFDANVLYPAPMRDALMQLAVTDLFKAKWTADIHREWIDALLRNEPHRERAALERTRDLMDRATRDCLVTGYEA
LVPALTLPDPDDRHVLAAAIVGRCDAIVTQNLKDFPPAALAPFGIETQHPDDFFRNQLSLAPGLVCSALRKVRARLKNPL
KSVDEYLAILTQQGLVATVADLEQFADLL

Nucleotide

• Length: 570 bp

>T142207 NZ_CP060453:c4711325-4711191 [Xanthomonas citri pv. citri]
ATGAAGCGTGCAATTGTGCTGCTGGTGCTGTCGATGTTGTCGGTGGGAATGCTGGCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGCAGGGCGCTGGCGAGAAGGTGGAAGACGCAGCGCGTAAGTAA

Antitoxin

Protein

• Length: 155 a.a.
• Molecular weight: 16891.48 Da
• Isoelectric Point: 6.4929

>AT142207 WP_007803221.1 NZ_CP045624:c61613-61149 [Roseibium aggregatum]
MNMLAHRHLPPTPQDAAIARVSGQALSRFAQARAPLKLRVTDSEQMEPIELPAGAVSLLMEILEAMAAGRGVTIIPENAE
LSTVQAAEVLNVSRPFLIKLLEDGAIPHRKVGKHRRVRMEDVMSYKAAIDTEREAALDQLAADAQEQDMGYRSK

Nucleotide

• Length: 465 bp

>AT142207 NZ_CP060453:c4711552-4711400 [Xanthomonas citri pv. citri]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTTTCGGCCGGCGTGATGACGGGCTGCAACACCGTGGCCGGCGC
CGGCAAGGACATGCAGGGTGCGGGCAACAAGGTCGAGAAAACGGCCGAAAAATGCAGCGACGGTAAGTGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A1U9Z9S0