Detailed information of TA system TA142186

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2366451..2367429
• Replicon: chromosome
• Accession: NZ_CP045606
• Organism: Bacillus cereus strain SB1

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: GE376_RS12100
• Protein ID: WP_153217846.1
• Coordinates: 2366451..2367188 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: M1PKC2
• Locus tag: GE376_RS12105
• Protein ID: WP_000237819.1
• Coordinates: 2367301..2367429 (+)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GE376_RS12080	2362103..2362875	+	773	Protein_2322	class I SAM-dependent methyltransferase	-
GE376_RS12085	2363033..2364718	-	1686	WP_198049018.1	alpha-keto acid decarboxylase family protein	-
GE376_RS12090	2364826..2365308	+	483	WP_000191889.1	MarR family winged helix-turn-helix transcriptional regulator	-
GE376_RS12095	2365475..2366212	+	738	WP_000594149.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
GE376_RS12100	2366451..2367188	+	738	WP_153217846.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
GE376_RS12105	2367301..2367429	+	129	WP_000237819.1	hypothetical protein	Antitoxin
GE376_RS31460	2367502..2367678	+	177	WP_000852631.1	hypothetical protein	-
GE376_RS12110	2367722..2367997	-	276	WP_001032898.1	hypothetical protein	-
GE376_RS31465	2368508..2368684	+	177	WP_000852630.1	hypothetical protein	-
GE376_RS12115	2368829..2370298	+	1470	WP_153217849.1	beta-Ala-His dipeptidase	-
GE376_RS12120	2370544..2371353	+	810	WP_000678370.1	C39 family peptidase	-
GE376_RS12125	2371379..2371981	+	603	WP_023521942.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28351.15 Da
• Isoelectric Point: 8.2998

>T142186 WP_153217846.1 NZ_CP045606:2366451-2367188 [Bacillus cereus]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAIFGTMEWHMEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T142186 NZ_CP060441:c1255014-1254838 [Citrobacter cronae]
GTGAAGCAAAGTGAGTTCAGACGCTGGCTTGAATCTCAGGGAGTTAAAGTTACAAACGGCTCAAATCATCTGAAGTTGAG
GTATCAGGGAAGGCGTAGCGTTATGCCCAGGCATCCTGTTGATGAGATAAAAGAGGCCCTGCGAAAAGCGATCCAAAAGC
AGCCTGGCTTACAGTAA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 4953.36 Da
• Isoelectric Point: 3.9798

>AT142186 WP_000237819.1 NZ_CP045606:2367301-2367429 [Bacillus cereus]
VVKDYDNDFFKEGNEDTTDFSLIKGDTLQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT142186 NZ_CP060441:c1254791-1254375 [Citrobacter cronae]
ATGCGTTATCCCGTTAATCTAATACCCGCCGTGGAAGGTGGGTTCATGGTGTCTTTTCCGGATATTCCTGAAGCATTGAC
TCAGGGAGAAACTCGCCATGATGCTTTGCAGGCTGCGCAGGCCGCATTGGTCACCGCTTTTGAATTCTATTTCGACGATA
ACGAACCGATTCCTTTGCCTTCAGCGGTTGTCGCTGGCGCTGACTATGTGGAAATACCACTGAGCGTTGCGTCTAAGGTT
CTGTTGTTAAATGCCTTCCTTGAATCGAATATTACCCAGCAGGAGTTGGCAAACAGGATCGGCAGGCCTAAGCAGGAAAT
CACCCGTTTATTCGATTTAAAGCACGCGACAAAAATTGATGCTGTGCAGATAGCTGCCCGCGCTTTAGGAAAAGAACTGT
CGGTAACGATGTTGTAA

Structures

Antitoxin

• AlphaFold DB: M1PKC2