Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 240256..240898 Replicon chromosome
Accession NZ_CP045606
Organism Bacillus cereus strain SB1

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag GE376_RS01355 Protein ID WP_000635965.1
Coordinates 240548..240898 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag GE376_RS01350 Protein ID WP_000004570.1
Coordinates 240256..240543 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GE376_RS01325 235573..236535 + 963 WP_000961144.1 UV DNA damage repair endonuclease UvsE -
GE376_RS01330 236528..237100 - 573 WP_000908526.1 rhomboid family intramembrane serine protease -
GE376_RS01335 237194..237553 + 360 WP_000583419.1 holo-ACP synthase -
GE376_RS01340 237710..238660 + 951 WP_002094231.1 outer membrane lipoprotein carrier protein LolA -
GE376_RS01345 238778..239947 + 1170 WP_000390593.1 alanine racemase -
GE376_RS01350 240256..240543 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
GE376_RS01355 240548..240898 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
GE376_RS01360 240967..243135 + 2169 WP_000426207.1 RNA-binding transcriptional accessory protein -
GE376_RS01365 243193..243309 - 117 WP_001143640.1 cortex morphogenetic protein CmpA -
GE376_RS01370 243505..243963 + 459 WP_097822610.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T142185 WP_000635965.1 NZ_CP045606:240548-240898 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T142185 NZ_CP060441:c1165343-1164861 [Citrobacter cronae]
ATGGAAATAAACGTCACGACACCGGTGTTGCTGACCGAAGGGCATGATCTCCAGCCATTTGATTGCGGTAATGACGTATT
AAATGATTGGCTACGTCGACGGGCGATTAAAAATCAGTACCTCAACGCATCGCGCACATTCGTCATTTGTCTTGAATCTA
CTCAGCGTGTTGTGGGTTACTATTCCATTGCAACCGGGTCCGTGAGCCATGCTGATTTAGGCCGTAGTTTACGTCAGAAT
ATGCCCGACCCGGTTCCGGTCGTTCTGCTGGGACGTCTTGCTGTTGATGTCTGTACTCAGGGGCATAGTTTCGGAAAATG
GCTGCTTAATGATGCGGTACTGCGAATTTCCAACTTAGCCGATCAAGTGGGCATCAAAGCCATCATGGTACATGCTATTG
ATGGCCAAGCTCGGGCATTCTATGAGTATTTCGGCTTCGTTCAGTCTCCGATAGCAGCAAATACGTTGTTCTACAAAATT
TAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT142185 WP_000004570.1 NZ_CP045606:240256-240543 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT142185 NZ_CP060441:c1165618-1165334 [Citrobacter cronae]
ATGCAAAGCAGCGCCCGTAAGCCGACTCGTGATAAGCAAATTAATATCCGGGCTACTGATGAGGAACGTGCGGTAATTGA
CTACGCTGCAAGTTTGGTCAATAAAAACCGTACTGACTTCATCATTGAGAAGGCCGTGCACGAGGCCCAGAATATCATTT
TAGATCAACGAGTATTCGTCCTTGACGATGCCCGTTATCAGTCTTTCATTGAACAGCTTGAAGCGCCGGTTCAGAACGTT
GAAGGGCGTCAACGCCTGATGAATGTGAAGCCCGAATGGAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References