Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RelE(toxin)
Location 1130862..1131355 Replicon chromosome
Accession NZ_CP045602
Organism Enterococcus faecium strain TK-P5D

Toxin (Protein)


Gene name relE Uniprot ID J6KIM0
Locus tag GFB66_RS05470 Protein ID WP_002400299.1
Coordinates 1131089..1131355 (+) Length 89 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A4Y3JLV3
Locus tag GFB66_RS05465 Protein ID WP_025478324.1
Coordinates 1130862..1131092 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GFB66_RS05440 1127111..1127878 + 768 WP_002400309.1 MurR/RpiR family transcriptional regulator -
GFB66_RS05445 1127879..1128322 + 444 WP_002400306.1 hypothetical protein -
GFB66_RS05450 1128531..1129121 - 591 WP_002400305.1 glutamine amidotransferase -
GFB66_RS05455 1129734..1130036 + 303 Protein_1081 hypothetical protein -
GFB66_RS05460 1130216..1130371 + 156 WP_002400302.1 hypothetical protein -
GFB66_RS05465 1130862..1131092 + 231 WP_025478324.1 hypothetical protein Antitoxin
GFB66_RS05470 1131089..1131355 + 267 WP_002400299.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GFB66_RS05475 1131606..1131834 + 229 Protein_1085 hypothetical protein -
GFB66_RS05480 1131850..1133016 + 1167 WP_002314829.1 oxygen-independent coproporphyrinogen III oxidase -
GFB66_RS05485 1133250..1134293 + 1044 WP_002294449.1 heat-inducible transcriptional repressor HrcA -
GFB66_RS05490 1134387..1134950 + 564 WP_002400298.1 nucleotide exchange factor GrpE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-87)

Antitoxin

Protein of unknown function DUF6290

(1-71)


Sequences

Toxin        


Download         Length: 89 a.a.        Molecular weight: 10642.41 Da        Isoelectric Point: 10.4982

>T142184 WP_002400299.1 NZ_CP045602:1131089-1131355 [Enterococcus faecium]
MTYRLEFTPEIQKQLRKMDKHQAMLIVRWLYQHIDGIDDPRKFGKGLTANRSGQWRYRIGKYRVLVEIEDKQLVVIAIQV
GHRRNIYD

Download         Length: 267 bp

>T142184 NZ_CP060441:c42628-42410 [Citrobacter cronae]
ATGTCTGATAAACCATTAACTAAAACAGATTATTTGATGCGCCTGCGACGCTGCCAGACAATTGACACGCTTGAGCGCGT
TATTGAAAAAAATAAATATGAATTGTCCGACAACGAACTGGCTGTATTTTACTCAGCAGCGGATCACCGTCTTGCTGAAT
TGACCATGAATAAGCTCTACGACAAGATTCCAACTTCCGTTTGGAAATTCATTCGCTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8790.97 Da        Isoelectric Point: 4.4723

>AT142184 WP_025478324.1 NZ_CP045602:1130862-1131092 [Enterococcus faecium]
MAMITVRVSDAEKEWLSYMAKFYGITLSELLKTYSMEQLEDEYDRQTAEIAHKHWVESGKTTVSMEEILHEFGGLG

Download         Length: 231 bp

>AT142184 NZ_CP060441:c43030-42656 [Citrobacter cronae]
ATGGATGAATACTCACCCAAAAGACATGATATCGCGCAGCTTAAATTTCTCTGCGAAACTCTGTATCATGACTGTCTTGC
AAACCTTGAACAAAGTAACCATGGCTGGGTTAACGACCCTACCTCTGCAACCAGCTTGCAGCTCAATGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAGGACAATAAGCTGATTGCGCAGATAGATGAATACCTG
GATGACACTTTTATGTTGTTCAGCAGTTATGGTATTAATACGCAGGATCTGCAGAAATGGCGGAAATCAGGTAATCGACT
GTTTCGCTGTTTTGTCAATGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4Y3JQ03

Antitoxin

Source ID Structure
AlphaFold DB A0A4Y3JLV3

References