142168

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 2626128..2626699 Replicon chromosome
Accession NZ_CP045598
Organism Enterococcus faecalis strain TK-P4B

Toxin (Protein)


Gene name mazF Uniprot ID R3GRA7
Locus tag GFB65_RS12440 Protein ID WP_002360937.1
Coordinates 2626128..2626469 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID R3JGB1
Locus tag GFB65_RS12445 Protein ID WP_002354773.1
Coordinates 2626469..2626699 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GFB65_RS12435 2622143..2625757 - 3615 WP_002389470.1 DNA-directed RNA polymerase subunit beta -
GFB65_RS12440 2626128..2626469 - 342 WP_002360937.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
GFB65_RS12445 2626469..2626699 - 231 WP_002354773.1 hypothetical protein Antitoxin
GFB65_RS12450 2626873..2627088 - 216 WP_002354768.1 zinc ribbon domain-containing protein -
GFB65_RS12455 2627227..2628219 + 993 WP_002358973.1 biotin--[acetyl-CoA-carboxylase] ligase -
GFB65_RS12460 2628483..2629136 - 654 WP_194848796.1 lytic polysaccharide monooxygenase -
GFB65_RS12465 2629717..2631333 + 1617 WP_002372620.1 phosphatase PAP2/LCP family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element ClpL - 2536148..2640860 104712


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13165.41 Da        Isoelectric Point: 9.3988

>T142168 WP_002360937.1 NZ_CP045598:c2626469-2626128 [Enterococcus faecalis]
MNVEKKYIPKKGDIVWIDFDPAAGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNIPTRYTLPDDIETHGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Download         Length: 342 bp

>T142168 NZ_CP060440:241852-242274 [Serratia marcescens]
GTGGCCCAGTGGCGATGTGATATCCGCATTTCCTGGCGCACTCAGCTGTTTTCCCTGTTGACCCACGGCGTTCTGATCCT
GCTGATCCTGATTTCGCCCTGGCCGGAAGGCTTGGGCCCGCTCTGGCTGGTGTTGCTGACGCTGGTGGTGTTTCAGTGCA
TTCGCAGCCAGAAGCGCATCGCCGCGGTGCAGGGCGAGCTGCGGCTGTTGGCGGATCGGCGCTTCAGCTGGCACGGCCGC
GAGTGGCGGCTGGCGAAAAAACCGTGGATGCCGGGTTACGGCATGCTGCTGACGCTGCAGCCGACGGAGGGCAAAAAGCG
CCGCCGGCTGTGGCTGGCTTCAGACTGCATGAGCAAAGAGGAGTGGCGCCACCTGCGGCAGCTGTTGCTGTATCCGTCGA
CGGGTGACGGCGAACCAGAGTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8677.67 Da        Isoelectric Point: 4.3313

>AT142168 WP_002354773.1 NZ_CP045598:c2626699-2626469 [Enterococcus faecalis]
MLTTKSRRQGSSVVLTLPTDNGKKPKVDQEYIVMYSDDGTITLVPKIQDPFSEGPEGEYYEKDEWHDLAPEGRELF

Download         Length: 231 bp

>AT142168 NZ_CP060440:241605-241871 [Serratia marcescens]
ATGGATATTAACAACAAAGCACGTATTCACTGGGCTTGCCGCCGTGGCATGCGCGAGCTGGACATCTCCATCATGCCGTT
CTTCGAATACGAATACGACAGCCTGAATGACGCGGACAAGGCGTTGTTCATCCGCCTGCTGGAGTGCGACGATCCCGATT
TGTTCAACTGGCTGATGAATCATGGCGCGCCGCAGGACAGCGAGCTGCAGAGAATGGTGACCCTGATCCAAACGCGAAAT
AAAGACCGTGGCCCAGTGGCGATGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M2A7G9

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2A812

References