Detailed information of TA system TA142154

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2598232..2599210
• Replicon: chromosome
• Accession: NZ_CP045585
• Organism: Bacillus thuringiensis strain x023

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: W8Y388
• Locus tag: GFH33_RS13750
• Protein ID: WP_000624977.1
• Coordinates: 2598232..2598969 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: W8YZL5
• Locus tag: GFH33_RS13755
• Protein ID: WP_000237818.1
• Coordinates: 2599082..2599210 (+)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GFH33_RS13730 (GFH33_13730)	2593875..2594657	+	783	WP_000381800.1	class I SAM-dependent methyltransferase	-
GFH33_RS13735 (GFH33_13735)	2594815..2596500	-	1686	WP_003284281.1	alpha-keto acid decarboxylase family protein	-
GFH33_RS13740 (GFH33_13740)	2596608..2597090	+	483	WP_000191888.1	MarR family winged helix-turn-helix transcriptional regulator	-
GFH33_RS13745 (GFH33_13745)	2597257..2597994	+	738	WP_000594152.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
GFH33_RS13750 (GFH33_13750)	2598232..2598969	+	738	WP_000624977.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
GFH33_RS13755 (GFH33_13755)	2599082..2599210	+	129	WP_000237818.1	hypothetical protein	Antitoxin
GFH33_RS13760 (GFH33_13760)	2599283..2599459	+	177	WP_000852629.1	stage II sporulation protein SB	-
GFH33_RS13765 (GFH33_13765)	2599478..2599867	-	390	WP_000713866.1	YxeA family protein	-
GFH33_RS13770 (GFH33_13770)	2600079..2601548	+	1470	WP_000287515.1	beta-Ala-His dipeptidase	-
GFH33_RS13775 (GFH33_13775)	2601794..2602603	+	810	WP_000678509.1	papain-like cysteine protease family protein	-
GFH33_RS13780 (GFH33_13780)	2602629..2603231	+	603	WP_000517260.1	hypothetical protein	-
GFH33_RS13785 (GFH33_13785)	2603471..2603722	-	252	WP_000827560.1	LuxR C-terminal-related transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28307.03 Da
• Isoelectric Point: 8.2998

>T142154 WP_000624977.1 NZ_CP045585:2598232-2598969 [Bacillus thuringiensis]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T142154 NZ_CP060420:c1242123-1241377 [Bacillus velezensis]
ATGCTTTTGTTTTTTCAGATCATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTGAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCTGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCAGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 5011.40 Da
• Isoelectric Point: 3.9150

>AT142154 WP_000237818.1 NZ_CP045585:2599082-2599210 [Bacillus thuringiensis]
VVKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT142154 NZ_CP060420:c1241376-1241206 [Bacillus velezensis]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Toxin

• AlphaFold DB: W8Y388

Antitoxin

• AlphaFold DB: W8YZL5