Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 238756..239398 Replicon chromosome
Accession NZ_CP045585
Organism Bacillus thuringiensis strain x023

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag GFH33_RS01355 Protein ID WP_000635965.1
Coordinates 239048..239398 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag GFH33_RS01350 Protein ID WP_000004570.1
Coordinates 238756..239043 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GFH33_RS01325 (GFH33_01325) 234073..235035 + 963 WP_000961167.1 UV DNA damage repair endonuclease UvsE -
GFH33_RS01330 (GFH33_01330) 235028..235600 - 573 WP_000908522.1 rhomboid family intramembrane serine protease -
GFH33_RS01335 (GFH33_01335) 235694..236053 + 360 WP_000583417.1 holo-ACP synthase -
GFH33_RS01340 (GFH33_01340) 236210..237160 + 951 WP_002100659.1 outer membrane lipoprotein carrier protein LolA -
GFH33_RS01345 (GFH33_01345) 237278..238447 + 1170 WP_000390616.1 alanine racemase -
GFH33_RS01350 (GFH33_01350) 238756..239043 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
GFH33_RS01355 (GFH33_01355) 239048..239398 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
GFH33_RS01360 (GFH33_01360) 239466..241634 + 2169 WP_000426236.1 Tex family protein -
GFH33_RS01365 (GFH33_01365) 241693..241809 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
GFH33_RS01370 (GFH33_01370) 242005..242463 + 459 WP_000344239.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T142153 WP_000635965.1 NZ_CP045585:239048-239398 [Bacillus thuringiensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T142153 NZ_CP060420:490254-490604 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT142153 WP_000004570.1 NZ_CP045585:238756-239043 [Bacillus thuringiensis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT142153 NZ_CP060420:489967-490248 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References