Detailed information of TA system TA142151

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 3184723..3185315
• Replicon: chromosome
• Accession: NZ_CP045571
• Organism: Acidithiobacillus thiooxidans ATCC 19377

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: A0A1C2IIL4
• Locus tag: GCD22_RS16675
• Protein ID: WP_031571551.1
• Coordinates: 3184977..3185315 (+)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: GCD22_RS16670
• Protein ID: WP_010639395.1
• Coordinates: 3184723..3184977 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GCD22_RS16640	3179819..3180247	-	429	WP_010639384.1	peptide-methionine (R)-S-oxide reductase MsrB	-
GCD22_RS16645	3180251..3180844	-	594	WP_031571565.1	peptide-methionine (S)-S-oxide reductase MsrA	-
GCD22_RS16650	3180953..3181534	+	582	WP_031571563.1	DUF4337 domain-containing protein	-
GCD22_RS16655	3181740..3182537	+	798	WP_031571559.1	thioredoxin fold domain-containing protein	-
GCD22_RS16660	3182591..3183556	-	966	WP_031571557.1	UDP-glucose 4-epimerase GalE	-
GCD22_RS16665	3183722..3184498	+	777	WP_035210251.1	hypothetical protein	-
GCD22_RS16670	3184723..3184977	+	255	WP_010639395.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
GCD22_RS16675	3184977..3185315	+	339	WP_031571551.1	type II toxin-antitoxin system ChpB family toxin	Toxin
GCD22_RS16680	3185554..3185868	+	315	WP_140391137.1	hypothetical protein	-
GCD22_RS16685	3186241..3186468	+	228	WP_031571547.1	hypothetical protein	-
GCD22_RS16695	3187611..3188468	-	858	WP_031571544.1	16S rRNA (cytidine(1402)-2'-O)-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-112)

Antitoxin

• SpoVT-AbrB domain (7-49)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12441.30 Da
• Isoelectric Point: 11.2221

>T142151 WP_031571551.1 NZ_CP045571:3184977-3185315 [Acidithiobacillus thiooxidans ATCC 19377]
MRWPRRGEIWQANLSPTSGREQQGHRPVLIVSREAFNRSGLVWVCPITQGGNQARFAGFAVSLMGSGTKTQGIVMSNQSR
TVDLMARDGKFVEMVPESIVNEVITRIQAILE

Nucleotide

• Length: 339 bp

>T142151 NZ_CP060417:518845-519195 [Bacillus subtilis subsp. subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGACGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9339.54 Da
• Isoelectric Point: 4.5488

>AT142151 WP_010639395.1 NZ_CP045571:3184723-3184977 [Acidithiobacillus thiooxidans ATCC 19377]
MNQTKIQKWGHSMALRIPATTIRSWGVGEGQAVSLEIENGALVARPAQKRYTLDELLSQCDFTQPISAEEREWIDAPSAG
IEEI

Nucleotide

• Length: 255 bp

>AT142151 NZ_CP060417:518559-518840 [Bacillus subtilis subsp. subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• AlphaFold DB: A0A1C2IIL4