Detailed information of TA system TA142147

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09812-MazE
• Location: 2521976..2522595
• Replicon: chromosome
• Accession: NZ_CP045571
• Organism: Acidithiobacillus thiooxidans ATCC 19377

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: GCD22_RS13290
• Protein ID: WP_081577346.1
• Coordinates: 2521976..2522329 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A5P9XTK8
• Locus tag: GCD22_RS13295
• Protein ID: WP_010642066.1
• Coordinates: 2522326..2522595 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GCD22_RS13260	2517347..2518960	-	1614	WP_153940819.1	diguanylate cyclase	-
GCD22_RS13265	2519052..2519267	-	216	WP_081577351.1	hypothetical protein	-
GCD22_RS13270	2519286..2519807	-	522	WP_081577350.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
GCD22_RS13275	2519913..2520443	-	531	WP_153940820.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
GCD22_RS13280	2520760..2521254	+	495	WP_153940821.1	sel1 repeat family protein	-
GCD22_RS13285	2521422..2521871	-	450	WP_170286749.1	nucleotidyltransferase domain-containing protein	-
GCD22_RS13290	2521976..2522329	-	354	WP_081577346.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
GCD22_RS13295	2522326..2522595	-	270	WP_010642066.1	AbrB family transcriptional regulator	Antitoxin
GCD22_RS13300	2522768..2522872	-	105	Protein_2588	PIN domain nuclease	-
GCD22_RS13305	2522872..2523111	-	240	WP_153940822.1	ribbon-helix-helix domain-containing protein	-
GCD22_RS13310	2523336..2524016	+	681	WP_081577345.1	hypothetical protein	-
GCD22_RS13315	2524086..2525171	-	1086	WP_065972780.1	IS630 family transposase	-
GCD22_RS13320	2525323..2525541	+	219	WP_153940823.1	hypothetical protein	-
GCD22_RS13325	2525683..2526885	+	1203	WP_170286750.1	site-specific integrase	-
GCD22_RS13330	2527103..2527300	+	198	WP_081577343.1	AlpA family phage regulatory protein	-
GCD22_RS13335	2527297..2527527	+	231	WP_081577342.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (9-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 12792.51 Da
• Isoelectric Point: 7.2439

>T142147 WP_081577346.1 NZ_CP045571:c2522329-2521976 [Acidithiobacillus thiooxidans ATCC 19377]
MIGRKIPSPGDVYWINTNPTSGNEIRDRHRVVVITPTEINKLGLVTCVPITSGGNHARQNGLTVVIAEHDTTGVAVCNQV
RTFDLSARAGEVKYIETLDQQTTREIINRVTSIIDPG

Nucleotide

• Length: 354 bp

>T142147 NZ_CP060414:1666690-1666872 [Neisseria musculi]
ATGAAATACAGTGAATTCAGAAAGTGGCTGCTCTCTCAAGGCGTAGAATTTACCACGCAGAAACGCGGCAGCCACCAACT
TATCAGATTAGGTAATAAAACATCGGTTTTCCCGAACCACGGCAGCAAAGAAATCGGCACGGGGCTGGTAAACAAAATCA
AAAAAGATTTAGATTTGAAATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9798.34 Da
• Isoelectric Point: 5.8471

>AT142147 WP_010642066.1 NZ_CP045571:c2522595-2522326 [Acidithiobacillus thiooxidans ATCC 19377]
MLITKIRKQGGAAVVTLPAEVLRQMDATIGEELSIEVSQHALIMRPARGERRRYSLGELLAGVTIQDMQALTENSAWARE
GEPVGRELL

Nucleotide

• Length: 270 bp

>AT142147 NZ_CP060414:1666925-1667341 [Neisseria musculi]
ATGTTGGCCTATCGCTATATTTTGACACCGGACGACAACGGCACTTTCTTGGTTACGTTTCCTGATATTCCGGAAGCGGT
TGCCGTGGGCGAAGATGAAGCATCTGCCGGAATCGAGGCGGCAGACGGCCTGATTTGCGCTTTGGAAGGTTATTTTGCAG
ACCGCCGCGAAGTGCCGCTGCCGTCTGGTAACGACGGGCGGCAGGTTGTGGTGTTGCCGGCATTGGAAACCGCCAAAGTG
CTGCTGCTGAACGAAATGCTGGCGCAAAATGTAAAAAAAGCAGAAATGGCGCGGCGTTTGGACGTGCATATGCCGCAAAT
CGACCGCCTGCTGGATTTGCGCCATAATACGAAAATCGATTTTTTGGAAAAAGCTGCCGGCAAGCTCGGCAAGCGTCTGA
ACATCAGCTTTTCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A5P9XTK8