Detailed information of TA system TA142143

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ParE-CopA/RHH(antitoxin)
• Location: 1959015..1959406
• Replicon: chromosome
• Accession: NZ_CP045571
• Organism: Acidithiobacillus thiooxidans ATCC 19377

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: GCD22_RS18205
• Protein ID: WP_202806246.1
• Coordinates: 1959293..1959406 (+)
• Length: 38 a.a.

Antitoxin (Protein)

• Gene name: copA
• Uniprot ID: -
• Locus tag: GCD22_RS10365
• Protein ID: WP_010638636.1
• Coordinates: 1959015..1959305 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GCD22_RS10340	1954692..1955057	-	366	WP_031575356.1	HTH domain-containing protein	-
GCD22_RS10345	1955059..1955352	-	294	WP_035211427.1	hypothetical protein	-
GCD22_RS10350	1955487..1956326	+	840	WP_031575351.1	prolipoprotein diacylglyceryl transferase	-
GCD22_RS10355	1956356..1958338	+	1983	WP_031575348.1	UvrD-helicase domain-containing protein	-
GCD22_RS10360	1958534..1958812	-	279	WP_010638638.1	30S ribosomal protein S20	-
GCD22_RS10365	1959015..1959305	+	291	WP_010638636.1	CopG family ribbon-helix-helix protein	Antitoxin
GCD22_RS18205	1959293..1959406	+	114	WP_202806246.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GCD22_RS18210	1959410..1959586	+	177	WP_202806247.1	type II toxin-antitoxin system RelE/ParE family toxin	-
GCD22_RS10375	1959608..1960624	-	1017	WP_031575342.1	ABC transporter ATP-binding protein	-
GCD22_RS10380	1960642..1961424	-	783	WP_031575339.1	ABC transporter permease	-
GCD22_RS10385	1961704..1962051	+	348	WP_031575336.1	DsrE family protein	-
GCD22_RS10390	1962056..1962892	+	837	WP_031575334.1	symmetrical bis(5'-nucleosyl)-tetraphosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-36)

Antitoxin

• Ribbon-helix-helix protein, CopG (9-46)

Sequences

Toxin

Protein

• Length: 38 a.a.
• Molecular weight: 4115.95 Da
• Isoelectric Point: 11.9906

>T142143 WP_202806246.1 NZ_CP045571:1959293-1959406 [Acidithiobacillus thiooxidans ATCC 19377]
MPQVKFAPAALRDLERLREFLRPKNPVAAKRAAAAIV

Nucleotide

• Length: 114 bp

>T142143 NZ_CP060404:5779700-5780077 [Streptomyces buecherae]
ATGAAGTTCCTGGACCTGCCCCAGTTGTTGGGGCTCGCCCAGCGTCTCGGGGAGAGCGAGGTGCGCGACTACGGATTGCT
GGAGTCCGCACTCGCTCGCCCCCAGGCCAGCGTGTTCGGCCAGGACGCGTACCCCGACGTGTGGCAGAAGGCCGCAGCGC
TGATGGAGTCGCTGGCCCGAAACCACGCCATGGTGGATGGCAACAAGCGCCTCGCCTGGTACGCGACCTGGGTCTTTCTG
CACGTCAACGGCCATCCGCTGGTCGAGGGCTTCGACGTGGACGAGGCTGAGCGCTTCGTCTTGAACGTGGCCCAGGGCGA
GCTGGACGTACCCAAGATCGCGGAATTGCTGCCGCGATTCGCGGCTTCCTCGGCGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 11286.56 Da
• Isoelectric Point: 5.5750

>AT142143 WP_010638636.1 NZ_CP045571:1959015-1959305 [Acidithiobacillus thiooxidans ATCC 19377]
MPATAAKTVSVKLDNDIRDRIRHLADVRHRTTHWIMRQAIEEYVEREEKREAFRQGTLKAWEEYQETGLHVTGDEVIAWL
ETWGDDNEQTAPACHK

Nucleotide

• Length: 291 bp

>AT142143 NZ_CP060404:5779503-5779700 [Streptomyces buecherae]
ATGGCCGCACTCAACCTGCGTTTCACGGAAGAAGAACTCGACGCCCTCAGAGAACGCGCGGAAGCTGAGGGGCGCAGCAT
GCAGGCGTTCGCTCACGACGCCGTGATGGTCGCCATAAACGAACACTCCCGGCTCTACAACGAGGCCGCCGATCACGTGC
TGAAGGTCAGCGCCGAGCTGAACCGGAGGCTCGCCTGA