Detailed information of TA system TA142141

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 1507430..1507965
• Replicon: chromosome
• Accession: NZ_CP045571
• Organism: Acidithiobacillus thiooxidans ATCC 19377

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: GCD22_RS07935
• Protein ID: WP_153940915.1
• Coordinates: 1507430..1507753 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: GCD22_RS07940
• Protein ID: WP_211371699.1
• Coordinates: 1507750..1507965 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GCD22_RS07910	1503691..1504281	-	591	WP_153940595.1	pilus assembly protein PilP	-
GCD22_RS07915	1504274..1504906	-	633	WP_153940596.1	type 4a pilus biogenesis protein PilO	-
GCD22_RS07920	1504910..1505476	-	567	WP_153940914.1	PilN domain-containing protein	-
GCD22_RS07925	1505478..1506695	-	1218	WP_153940597.1	type IV pilus assembly protein PilM	-
GCD22_RS07930	1506590..1507234	-	645	WP_153940598.1	hypothetical protein	-
GCD22_RS07935	1507430..1507753	-	324	WP_153940915.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
GCD22_RS07940	1507750..1507965	-	216	WP_211371699.1	antitoxin MazE family protein	Antitoxin
GCD22_RS07945	1508323..1508676	-	354	WP_153940600.1	hypothetical protein	-
GCD22_RS07950	1508794..1509468	-	675	WP_153940601.1	hypothetical protein	-
GCD22_RS07955	1509793..1510350	+	558	WP_081576904.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
GCD22_RS07960	1510360..1511010	+	651	WP_031574805.1	hypothetical protein	-
GCD22_RS07965	1511027..1512301	+	1275	WP_153940602.1	type II secretion system F family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-103)

Antitoxin

• Antitoxin MazE-like (8-70)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11607.55 Da
• Isoelectric Point: 10.0946

>T142141 WP_153940915.1 NZ_CP045571:c1507753-1507430 [Acidithiobacillus thiooxidans ATCC 19377]
VKRGAVVVVALHGDYGKPRPALVVQSEAFSALSSVTVLPITSDLRDAPLYRINVEASPETGLHKPSQIMVDKSQTVPREK
IGQVVGYLDYRKMVAVNRAMALFLGFA

Nucleotide

• Length: 324 bp

>T142141 NZ_CP060402:c3096655-3096401 [Microbacterium sp. YJN-G]
ATGCGTCTCGTCTGGGACAGGTCCGCCTGGGAGGACTATGTGCACTGGCAGACGGCCGACCGGAGAGTGCTCAAACGCAT
CAACGCCCTTATCGACGCATGCCTGCGTGATCCTTTCGAAGGCATCGGCAAGCCTGAGCAGCTGAAGTATGGCGCTCAGG
GTGCCTGGTCTCGACGCATAACCGAGGAGCATCGCCTTGTCTATCTCGTCGAGCGTGATGACCTCATCGTGCTGCAGGCG
CGCTACCACTACTGA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 8235.42 Da
• Isoelectric Point: 8.5675

>AT142141 WP_211371699.1 NZ_CP045571:c1507965-1507750 [Acidithiobacillus thiooxidans ATCC 19377]
VTTVHANTRVKKHRDALRAAGLRPIQIWVPDTRRPGFVEECRRQCLVLRNDPQEKEIMNWLEAAADTDGWV

Nucleotide

• Length: 216 bp

>AT142141 NZ_CP060402:c3096906-3096655 [Microbacterium sp. YJN-G]
ATGTCCATTACGGCCAGTGAGGCTCGCAAGACGCTTTTCCCGTTGATCGAGCGGGTCAATGCCGATCGAGACGCCGTGGA
GATCGTGTCCCGGAAGGGCAATGCCGTCCTGATGCCTGCCGACGAGTATGCGGCGTGGCAGGAGACGGCGTACCTGTTCC
GGTCGCCCGCGAACGCTCGCCGACTGCTCGACGCCTATGAGCGAGCGCGCGCCGGCGAAGTCGAGGAGCACGAACTCGAC
CGAGACGAATGA