Detailed information of TA system TA142137

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 577378..577979
• Replicon: chromosome
• Accession: NZ_CP045571
• Organism: Acidithiobacillus thiooxidans ATCC 19377

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: GCD22_RS03105
• Protein ID: WP_153940389.1
• Coordinates: 577782..577979 (-)
• Length: 66 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: GCD22_RS03100
• Protein ID: WP_153940388.1
• Coordinates: 577378..577785 (-)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GCD22_RS03070	572923..574473	+	1551	WP_153940899.1	relaxase/mobilization nuclease domain-containing protein	-
GCD22_RS03075	574753..575049	+	297	WP_153940384.1	type II toxin-antitoxin system RelE/ParE family toxin	-
GCD22_RS03080	575053..575352	+	300	WP_012536655.1	putative addiction module antidote protein	-
GCD22_RS03085	575452..575766	+	315	WP_153940385.1	hypothetical protein	-
GCD22_RS03090	575918..576370	+	453	WP_153940386.1	hypothetical protein	-
GCD22_RS03095	576367..577335	+	969	WP_153940387.1	P-type conjugative transfer ATPase TrbB	-
GCD22_RS03100	577378..577785	-	408	WP_153940388.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
GCD22_RS03105	577782..577979	-	198	WP_153940389.1	type II toxin-antitoxin system HicA family toxin	Toxin
GCD22_RS03110	578261..578647	+	387	WP_153940390.1	TrbC/VirB2 family protein	-
GCD22_RS03115	578651..579028	+	378	WP_153940391.1	VirB3 family type IV secretion system protein	-
GCD22_RS03120	579028..581562	+	2535	WP_153940392.1	conjugal transfer protein TrbE	-
GCD22_RS03125	581565..581795	+	231	Protein_603	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	543342..644627	101285

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-126)

Sequences

Toxin

Protein

• Length: 66 a.a.
• Molecular weight: 7183.31 Da
• Isoelectric Point: 9.8602

>T142137 WP_153940389.1 NZ_CP045571:c577979-577782 [Acidithiobacillus thiooxidans ATCC 19377]
MTSSELIKCLESAGWLHRSTKGSHHVYVHPDHPGHLSIPHPRKDLGTGLLHKLLKQAGLKEGNCK

Nucleotide

• Length: 198 bp

>T142137 NZ_CP060401:4234436-4234744 [Xenorhabdus nematophila]
ATGCAATTTTTTGTTTATCAATACAAACGTAGTGGAAGTAAATACAGCATGTTTGTCGATGTCCAGAGTGATATCATTGA
AACATTAGGAAGGCGGATCGTTATTCCTTTGATTGAAGCAAATCATTTCTCAAATAAAGTCAATCGTCAGTTATTCCCTG
TAGTGCAACTCAGAGGTGAGAATTACCGGCTTTTAACAACTGAAATTTCAGGTGTGGCTGAAAGTGTCATAGGTCACGAA
ATTGCTGATGTAAACCCATTCTCATCAGATATTAAGAACGCGCTTAATATCTTGTTTTGGGGCGTTTAA

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 14957.85 Da
• Isoelectric Point: 4.4429

>AT142137 WP_153940388.1 NZ_CP045571:c577785-577378 [Acidithiobacillus thiooxidans ATCC 19377]
MRYPIAIESEDETHAFGVVVPDLPGCFSAGDTLDEAIQMAKEAIELWLEVQIDEGGDIPEPKSITEHQANPEFAHWIWAL
VDVDPAILSDKAERVNITLPARVLRRVDEAAKSRHESRSGFLARAALATLRREAS

Nucleotide

• Length: 408 bp

>AT142137 NZ_CP060401:4234217-4234435 [Xenorhabdus nematophila]
ATGAAACATAGAGTTAATGTAACAGTTGATAAAGAGAATTATCTCATCCTGAGTTCTGCCGGGGTCAATATTTCAGGTTT
TGTTAATGAGCTCATGAGCAAGGAAGCTCAACGTATCAAAGCCGAGAATTGGAAAAAAGATAATTTGGAAGGCATGGAAG
AAGTGGCTCAATTTATCAAACAGCATGGTTCATTTGCTGATGAAAACAGGGACTGGTAA