Detailed information of TA system TA142136

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 574753..575352
• Replicon: chromosome
• Accession: NZ_CP045571
• Organism: Acidithiobacillus thiooxidans ATCC 19377

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: GCD22_RS03075
• Protein ID: WP_153940384.1
• Coordinates: 574753..575049 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: GCD22_RS03080
• Protein ID: WP_012536655.1
• Coordinates: 575053..575352 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GCD22_RS03050	570440..570718	+	279	WP_153940380.1	hypothetical protein	-
GCD22_RS03055	570729..571100	+	372	WP_153940381.1	hypothetical protein	-
GCD22_RS03060	571123..572067	+	945	WP_153940382.1	hypothetical protein	-
GCD22_RS03065	572132..572923	+	792	WP_153940383.1	hypothetical protein	-
GCD22_RS03070	572923..574473	+	1551	WP_153940899.1	relaxase/mobilization nuclease domain-containing protein	-
GCD22_RS03075	574753..575049	+	297	WP_153940384.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GCD22_RS03080	575053..575352	+	300	WP_012536655.1	putative addiction module antidote protein	Antitoxin
GCD22_RS03085	575452..575766	+	315	WP_153940385.1	hypothetical protein	-
GCD22_RS03090	575918..576370	+	453	WP_153940386.1	hypothetical protein	-
GCD22_RS03095	576367..577335	+	969	WP_153940387.1	P-type conjugative transfer ATPase TrbB	-
GCD22_RS03100	577378..577785	-	408	WP_153940388.1	type II toxin-antitoxin system HicB family antitoxin	-
GCD22_RS03105	577782..577979	-	198	WP_153940389.1	type II toxin-antitoxin system HicA family toxin	-
GCD22_RS03110	578261..578647	+	387	WP_153940390.1	TrbC/VirB2 family protein	-
GCD22_RS03115	578651..579028	+	378	WP_153940391.1	VirB3 family type IV secretion system protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	543342..644627	101285

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (48-92)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11088.84 Da
• Isoelectric Point: 10.0405

>T142136 WP_153940384.1 NZ_CP045571:574753-575049 [Acidithiobacillus thiooxidans ATCC 19377]
MISIKTTATFDRWLHGLRDRRARARVQVRIDRMELAHFGDCEPVGEGVSEMRIHYGPGYRVYFKRFGLELVVLLAGGDKS
TQSADIALALKLARQLEG

Nucleotide

• Length: 297 bp

>T142136 NZ_CP060401:3779441-3780739 [Xenorhabdus nematophila]
ATGAACAAATTGATCGTTGCATTGAATGGGCTGGAAGTGGGGGTCTTGTTTCGTGATAAAAAAGGAGCCATGTCTTTCCA
GTACACAAATGAGTGGCTGAAAGAACCCGGTACTCGTGCAATATCACTTTCTTTACCTCTCAGTAACCAGAAATATGTAG
GTCAGGAAGTATTCAATTTCTTCGATAATTTATTACCCGATTCAAGGGAAATCATAGCCCGTATGCAGGCTAGATTTCAG
ATAACCAGTAAGCATCCATTCGATCTCTTAGCCAGTGTTGGGAGGGACTGTGTTGGCGCTATTCAGCTCTATCAGGAAGG
TAATCAAATTCCTCCAATTGACGAGGTTCACGCTGAGCCTTTGGATGAGCGGGCAATAGTCGAGTTGCTTGAAGGCTACA
AGACGACGCCTTTAGGGATGAACAATAGTGACAATTTCCGGATCTCTTTGGCTGGCGCGCAGGAAAAAACAGCCCTTCTA
TGGCATCAAGGCTGTTGGCAGCGACCTTACGGGAGTACGCCTACGAGTCATATATTTAAACTCCCCATAGGCAGAATTGA
GCACAATAATATCGATCTTCACGAGAGTTGTGAAAATGAATGGTTGTGTTTAAAAATCGCCCGAACATTCGGTCTTCCTG
TTGCAAATGCAGAGTTAGCGAAGTTTGGAGAAAAGACAGTATTGATTGTCGAACGTTTTGATCGCCGTTGGTCTTGTGAT
AATCGGTGGTTGATGCGTCTTCCTCAAGAGGATATGTGTCAGGCTATGGGGATTGCTCCTGCTCTTAAATATGAAGCAGA
TGGAGGTCCTGGGATTGCAGATATCATGACGTTGCTACTCGGCTCCAGAAACTCAATAACAGATCGAGAAACGTTTTTTA
AATCCCAAATTCTATATTGGTTATTGGCTGCGATAGATGGACATGGCAAAAATTTCAGCATTTTTATCGAGCCAGATTCG
TCTTACTCGATGACTCCTCTTTATGATATTTTGTCAGCTTATCCTATCTTTGGTGCTAAGGGAGTTGCTCCAAAGAAAGC
AAAAATGGCGATGGCTTTACGTGGTAAGAGTCGCCAGTATCATTGGGCTACCATTCAACCCAGACATTTTCCAAGTACTG
CTCGTCAGGTTGGTTTTTCTGAATCAATGGCTGAAAAGCTGATGAACGAGATGGGAAATATGGCCGAAAAGGTAATAAAC
CAAGTAGCTAATGAGCTGCCACCTAATTTTCCCGGTCATATCAGCGATGCAATTTTTACTGGTTTGGCTAAACAAGCGGG
CAGGCTTTGTGCAGGGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10699.15 Da
• Isoelectric Point: 5.5858

>AT142136 WP_012536655.1 NZ_CP045571:575053-575352 [Acidithiobacillus thiooxidans ATCC 19377]
MEKLELHTWDSAEHLKTEEDIAAYLDACFEEAGDDAAFIARALGTVARARGMTRLAHDAGMGRESLYKALSGEGNPSFAT
ILKVAKALGVRIRAEALHV

Nucleotide

• Length: 300 bp

>AT142136 NZ_CP060401:3779189-3779440 [Xenorhabdus nematophila]
ATGAAGATCACATCACCACAAACACTGGCTGTAGCAATCAGGGAACAGCGCAAAAAAAAGCGCCTGACCCAAAAAGACAC
TGCTGATCGGGTTGGAATAAAGCAGAGTACCGTTTCTGGATTTGAAAACAGTCCGGAAAAAAGCAAGCTGGAAACCTTGT
TTAAATTGCTTTCTTCGCTAGGTTTGGAACTGCAAGTTACAGAGCGCAATGGTTCAACAAAGTCAGATAGTCGCTGGACA
AGGGAGTGGTAA