Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2618221..2618872 Replicon chromosome
Accession NZ_CP045567
Organism Lacticaseibacillus paracasei strain TK-P4A

Toxin (Protein)


Gene name pemK Uniprot ID K6S8L5
Locus tag GFB64_RS12650 Protein ID WP_003567661.1
Coordinates 2618221..2618604 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID K6RKI5
Locus tag GFB64_RS12655 Protein ID WP_003567665.1
Coordinates 2618624..2618872 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GFB64_RS12645 2617516..2618037 - 522 WP_003567657.1 QueT transporter family protein -
GFB64_RS12650 2618221..2618604 - 384 WP_003567661.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
GFB64_RS12655 2618624..2618872 - 249 WP_003567665.1 antitoxin Antitoxin
GFB64_RS12660 2618940..2620076 - 1137 WP_016369534.1 alanine racemase -
GFB64_RS12665 2620063..2620437 - 375 WP_003567669.1 holo-ACP synthase -
GFB64_RS12670 2620607..2622115 - 1509 WP_003567670.1 DEAD/DEAH box helicase -
GFB64_RS12675 2622241..2622384 - 144 WP_003567673.1 hypothetical protein -
GFB64_RS12680 2622415..2623803 - 1389 WP_003567677.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13773.03 Da        Isoelectric Point: 10.9764

>T142135 WP_003567661.1 NZ_CP045567:c2618604-2618221 [Lacticaseibacillus paracasei]
MDVSVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSTKMAAVDRALAISVGLVSLPKPKTYNKN

Download         Length: 384 bp

>T142135 NZ_CP060401:3447530-3447841 [Xenorhabdus nematophila]
ATGCACGTTATTTCTAAGGAACCCTTTGAGCAAGCTGTTCGTGATTATCCCAATGATGCAAACGTCCTTATCACGACGTA
TAAGCTGTTAAAAGAAAAGAATTTTGATAGCCCGTTGGAATTACAAAAACTGTTTCCGAGTCTTGATAATTTTAAGCACA
GGAACAAATGGTGGGTAATCGACATAGGTGGCAATAACTTACGGATGATTGCCTATATCAACTTTATGAACAAACGCTTT
TACGTTAAGCACATCGTCACTCACAAAGAATATGACAAATTGACAAAGAAGTATCGGGAGACAAAAGAATGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8938.08 Da        Isoelectric Point: 4.4610

>AT142135 WP_003567665.1 NZ_CP045567:c2618872-2618624 [Lacticaseibacillus paracasei]
MKKQTSVAVTFDDAIKARLDAYCELNDIDAQAVITEAVATFLKSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Download         Length: 249 bp

>AT142135 NZ_CP060401:3447838-3448257 [Xenorhabdus nematophila]
ATGATAAGAGAACCTATCAAAGCCCTTCAAGCGACCACGGCTCTGATCGCTGCTGTTCCCTTTTTAGGGAGCAGTGCTTC
TGAAAAGGATTATCGGGATGCACTGGCGATGGTTGATTATCTCATCGAAAACCACGATGAAAGCCCATTAATCAATATAC
TGGCCGCAAAAATAGCGGAGTATGAAGACAGTAACAAACGTTTTGCTGCCTTTAACAAAGCGGTGGAAGTGATGCCAACG
GGTGTCGCCGCGCTGCGTGTATTGATGGATCAACATGGCTTGTCATATTCAGATTTGCAAGAAGAAATAGGCTCAAAGTC
ACTGATCAGCCAAATTTTTTCGGGAAAACGAGCATTAACCATTGCACATATCAAGGCACTTTCTGCCCGTTTTGATGTGA
AGCCGGATATCTTTCTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K6S8L5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2BNY2

References