Detailed information of TA system TA142133

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higB-toxA/Tad-HTH_37
• Location: 432970..433598
• Replicon: chromosome
• Accession: NZ_CP045567
• Organism: Lacticaseibacillus paracasei strain TK-P4A

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S2NVS0
• Locus tag: GFB64_RS02000
• Protein ID: WP_016373562.1
• Coordinates: 433230..433598 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: toxA
• Uniprot ID: -
• Locus tag: GFB64_RS01995
• Protein ID: WP_003583264.1
• Coordinates: 432970..433230 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GFB64_RS01970	428861..429604	+	744	WP_194853818.1	SDR family oxidoreductase	-
GFB64_RS01975	429717..430343	+	627	WP_003583257.1	flavodoxin	-
GFB64_RS01980	430594..431097	+	504	WP_003583259.1	TetR/AcrR family transcriptional regulator	-
GFB64_RS01985	431102..432163	+	1062	WP_003583262.1	ABC transporter permease	-
GFB64_RS01990	432168..432857	+	690	WP_003593002.1	ABC transporter ATP-binding protein	-
GFB64_RS01995	432970..433230	-	261	WP_003583264.1	helix-turn-helix transcriptional regulator	Antitoxin
GFB64_RS02000	433230..433598	-	369	WP_016373562.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GFB64_RS02005	433890..435260	-	1371	WP_165846935.1	FAD-dependent oxidoreductase	-
GFB64_RS02010	435624..436442	-	819	WP_042745720.1	Cof-type HAD-IIB family hydrolase	-
GFB64_RS02015	436759..437028	-	270	WP_003563382.1	type II toxin-antitoxin system YafQ family toxin	-
GFB64_RS02020	437269..438276	+	1008	WP_194853819.1	LacI family DNA-binding transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (34-112)

Antitoxin

• Cro/C1-type helix-turn-helix domain (28-79)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 14923.01 Da
• Isoelectric Point: 8.3986

>T142133 WP_016373562.1 NZ_CP045567:c433598-433230 [Lacticaseibacillus paracasei]
MYKIDFYEDRDGYSEIEDFLDQLRHSHQKNNVSLLNKITKELYMLQNLGPRLREPHAKFLKGYAYPIMELRPMPERIFYA
AWQKDHFVLLHHYTKKTNKTDHREILRAQANLEDWLSRKEEN

Nucleotide

• Length: 369 bp

>T142133 NZ_CP060401:3431748-3432137 [Xenorhabdus nematophila]
ATGTATATGTTGGACACAAACACGGTCAGCTATATTTTCCGTCAAAATCAGGCGGTAACTGCTAAATTGCGAACCATACC
ACCATCAGAGATATGTATTTCCAGTATCACTGAAGCTGAGTTGCGTTTTGGTTTGGCTAAACGTCAAAATCACGCATTAG
AACAAGTCGTAAATACATTTATTGATTCAATCACTGTTTACGACTGGGATCAAAACGCTGCCAAAACCTATGGGACATTG
CGAGCCGGCATGGAACAGACTGGCCGAACTATGGGGACAATGGATCAACTGATTGCAGCACATGCACTGAGCCGAGAATT
AACTCTCGTTACCAGCGATTCTGCGTTTGAAATGGTTATTGGATTAAATATCGAAAATTGGGCGAAATAG

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9828.45 Da
• Isoelectric Point: 10.2439

>AT142133 WP_003583264.1 NZ_CP045567:c433230-432970 [Lacticaseibacillus paracasei]
MTTWEDFSQKLKKDNPEKLAIIKTLADLESERIKQGISQKDFAEKIGMKQPQLAKIERLDSLPTLATLNRYARGLGKEIR
LSIVTQ

Nucleotide

• Length: 261 bp

>AT142133 NZ_CP060401:3431485-3431748 [Xenorhabdus nematophila]
ATGGAACGAATTGCAAAAATCTTCAAAAACGGACGCAATCAGGCTGTCAGGCTGCCTGTTGCGTTTGAATTTGATACTGA
CCAGGTTTATATCCGAAAAAATGAGAACGGAGACGTTATTCTCTCCAAAAACAGACCGTCTACTGACAATTGGGATAATT
TTTTCAATATGCTCGGTGCTGTATCTGTTCCTGATAATTTTCTGGATGCAAATGATCGTAACCAGGATGACACAGAACGT
GACCCATTCGAGGACGTTTTCTGA

Structures

Toxin

• AlphaFold DB: S2NVS0