Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | BrnTA/BrnT-BrnA |
| Location | 161466..162054 | Replicon | plasmid pAC1530 |
| Accession | NZ_CP045561 | ||
| Organism | Acinetobacter nosocomialis strain AC1530 | ||
Toxin (Protein)
| Gene name | brnT | Uniprot ID | N8SM64 |
| Locus tag | GD578_RS19995 | Protein ID | WP_000438825.1 |
| Coordinates | 161767..162054 (-) | Length | 96 a.a. |
Antitoxin (Protein)
| Gene name | brnA | Uniprot ID | D3JXQ4 |
| Locus tag | GD578_RS19990 | Protein ID | WP_004728120.1 |
| Coordinates | 161466..161780 (-) | Length | 105 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| GD578_RS19960 | 156566..156934 | - | 369 | Protein_150 | IS1-like element ISAba3 family transposase | - |
| GD578_RS19965 | 157015..157857 | + | 843 | WP_002002480.1 | carbapenem-hydrolyzing class D beta-lactamase OXA-58 | - |
| GD578_RS19970 | 157911..158939 | + | 1029 | WP_081106503.1 | transposase | - |
| GD578_RS19975 | 159054..159806 | + | 753 | Protein_153 | IS1-like element ISAba3 family transposase | - |
| GD578_RS19980 | 159934..160761 | + | 828 | WP_000073658.1 | AraC family transcriptional regulator | - |
| GD578_RS19985 | 160811..161416 | + | 606 | WP_000447788.1 | LysE family translocator | - |
| GD578_RS19990 | 161466..161780 | - | 315 | WP_004728120.1 | BrnA antitoxin family protein | Antitoxin |
| GD578_RS19995 | 161767..162054 | - | 288 | WP_000438825.1 | BrnT family toxin | Toxin |
| GD578_RS20000 | 162326..162856 | - | 531 | WP_000172759.1 | hypothetical protein | - |
| GD578_RS20005 | 163005..163307 | + | 303 | Protein_159 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| GD578_RS20010 | 163308..163589 | + | 282 | WP_000985609.1 | putative addiction module antidote protein | - |
| GD578_RS20015 | 163669..164088 | - | 420 | WP_001180106.1 | SMI1/KNR4 family protein | - |
| GD578_RS20020 | 164230..164550 | + | 321 | WP_000897307.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| GD578_RS20025 | 164543..164815 | + | 273 | WP_000369781.1 | helix-turn-helix domain-containing protein | - |
| GD578_RS20030 | 165308..166783 | + | 1476 | WP_000052512.1 | ABC-F type ribosomal protection protein Msr(E) | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | aac(3)-IId / sul2 / blaNDM-1 / aph(3'')-Ib / aph(6)-Id / blaOXA-58 / msr(E) / mph(E) | - | 1..173972 | 173972 | |
| - | inside | IScluster/Tn | aph(3'')-Ib / aph(6)-Id / blaOXA-58 | - | 147542..159491 | 11949 | |
| - | flank | IS/Tn | msr(E) / mph(E) | - | 165308..169742 | 4434 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 96 a.a. Molecular weight: 11304.70 Da Isoelectric Point: 5.6205
>T142131 WP_000438825.1 NZ_CP045561:c162054-161767 [Acinetobacter nosocomialis]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTDGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTDGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG
Download Length: 288 bp
>T142131 NZ_CP060401:c3325780-3325472 [Xenorhabdus nematophila]
GTGTATAAGAGACAGGTATTCAGTAGGGAACGGGCAGAGTTGTTAACCGACGATGAATTTAGACACTTACAGGAACATTT
ACTAAAGAATCATGAACAAGGCAGTACCATTAGTGCTACTGGAGGATGCAAAAAGATTCGCTTGCAGCGGGAAGGAATGG
GAAAACGTGGCGGTGTCAGAATTATCTACTACACCATCATTAAAACCGGAATGCTCTATCTTCTTTTGATTTACCCTAAA
AATAAAAAAGATGACTTAACCCCAAAACAAAAAGAAGTACTTAAAGCCATCAGTAATAAATTGCAATAA
GTGTATAAGAGACAGGTATTCAGTAGGGAACGGGCAGAGTTGTTAACCGACGATGAATTTAGACACTTACAGGAACATTT
ACTAAAGAATCATGAACAAGGCAGTACCATTAGTGCTACTGGAGGATGCAAAAAGATTCGCTTGCAGCGGGAAGGAATGG
GAAAACGTGGCGGTGTCAGAATTATCTACTACACCATCATTAAAACCGGAATGCTCTATCTTCTTTTGATTTACCCTAAA
AATAAAAAAGATGACTTAACCCCAAAACAAAAAGAAGTACTTAAAGCCATCAGTAATAAATTGCAATAA
Antitoxin
Download Length: 105 a.a. Molecular weight: 12200.86 Da Isoelectric Point: 8.4628
>AT142131 WP_004728120.1 NZ_CP045561:c161780-161466 [Acinetobacter nosocomialis]
MSMVRYSRKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSSAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ
MSMVRYSRKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSSAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ
Download Length: 315 bp
>AT142131 NZ_CP060401:c3325426-3325130 [Xenorhabdus nematophila]
ATGGACAAAAAACTTTTTGATGATTTGATTGAAAGCATGAATCAAATGGTAGCGATTGATAAAGGGGAACTTTCTGTTCC
TGCTGAAAATATTCATCGTCATCGTCTGCCTGATGTAAAAAAACTACGCGAAACCTTTGGGTTAAAGCAAAGTGAATTTG
CCGAAGCTGTTGGCGTTTCTGCATCCTTGATCCAAAGCTGGGAGCAACACCGCCGTACTCCTTCTGGTTCATCACTTAAA
TTGCTTAAAATGCTTGAACGTAATCCTGCGCTGATTAATGAATTAAGCGCGCTTTAA
ATGGACAAAAAACTTTTTGATGATTTGATTGAAAGCATGAATCAAATGGTAGCGATTGATAAAGGGGAACTTTCTGTTCC
TGCTGAAAATATTCATCGTCATCGTCTGCCTGATGTAAAAAAACTACGCGAAACCTTTGGGTTAAAGCAAAGTGAATTTG
CCGAAGCTGTTGGCGTTTCTGCATCCTTGATCCAAAGCTGGGAGCAACACCGCCGTACTCCTTCTGGTTCATCACTTAAA
TTGCTTAAAATGCTTGAACGTAATCCTGCGCTGATTAATGAATTAAGCGCGCTTTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0A8TVQ4 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0A8TRI9 |