Detailed information of TA system TA142130

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 153684..154272
• Replicon: plasmid pAC1530
• Accession: NZ_CP045561
• Organism: Acinetobacter nosocomialis strain AC1530

Toxin (Protein)

• Gene name: relE
• Uniprot ID: N9CKJ9
• Locus tag: GD578_RS19940
• Protein ID: WP_004647749.1
• Coordinates: 153684..153980 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: GD578_RS19945
• Protein ID: WP_004969304.1
• Coordinates: 153982..154272 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GD578_RS19910	148734..149537	+	804	WP_001082319.1	aminoglycoside O-phosphotransferase APH(3'')-Ib	-
GD578_RS19915	149537..150373	+	837	WP_000480968.1	aminoglycoside O-phosphotransferase APH(6)-Id	-
GD578_RS19920	150515..150736	+	222	Protein_142	hypothetical protein	-
GD578_RS19925	150787..151491	-	705	WP_001067784.1	IS6-like element IS1006 family transposase	-
GD578_RS19930	151554..151946	+	393	Protein_144	recombinase family protein	-
GD578_RS19935	152167..153240	+	1074	WP_043041639.1	hypothetical protein	-
GD578_RS19940	153684..153980	+	297	WP_004647749.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GD578_RS19945	153982..154272	+	291	WP_004969304.1	putative addiction module antidote protein	Antitoxin
GD578_RS19950	154383..155837	-	1455	WP_026438532.1	SulP family inorganic anion transporter	-
GD578_RS19955	156087..156296	-	210	WP_004976362.1	hypothetical protein	-
GD578_RS19960	156566..156934	-	369	Protein_150	IS1-like element ISAba3 family transposase	-
GD578_RS19965	157015..157857	+	843	WP_002002480.1	carbapenem-hydrolyzing class D beta-lactamase OXA-58	-
GD578_RS19970	157911..158939	+	1029	WP_081106503.1	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(3)-IId / sul2 / blaNDM-1 / aph(3'')-Ib / aph(6)-Id / blaOXA-58 / msr(E) / mph(E)	-	1..173972	173972
	-	inside	IScluster/Tn	aph(3'')-Ib / aph(6)-Id / blaOXA-58	-	147542..159491	11949

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-92)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11584.25 Da
• Isoelectric Point: 5.8801

>T142130 WP_004647749.1 NZ_CP045561:153684-153980 [Acinetobacter nosocomialis]
MIEIKRLPEFDEWLDGIKDNMTRIRLNRRLDKVQRGNWGDIKPLQDGVWEMREFFGSGWRMYYIQHGDVVIVMLGGGDKS
TQQQDIDRAVKLSKTLED

Nucleotide

• Length: 297 bp

>T142130 NZ_CP060401:3322843-3323016 [Xenorhabdus nematophila]
GTGAAGCAAAGCGAGTTTAGACGGTGGCTGGAAGCTCAAGGTGTTGAAGTTTCAAACGGGACAAACCATCTGAAACTGAA
ATATAAGGGGAGACGAAGCATTATGCCGAGACACCCCAGCCAAGAGATAAAAGAACCACTGAGGAAAGCAATTCTTAAGC
AATTAGGATTGTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10524.19 Da
• Isoelectric Point: 5.1133

>AT142130 WP_004969304.1 NZ_CP045561:153982-154272 [Acinetobacter nosocomialis]
MVKVADLPSFDMAESLKTEEDIVMYLNMVLEENDPAELAHALGVIAKARGMTQIAKEAGIGREALYKALRQDSAPRFDTI
NRVVNALGLKLTVQHA

Nucleotide

• Length: 291 bp

>AT142130 NZ_CP060401:3323071-3323487 [Xenorhabdus nematophila]
ATGCGATATCCAGTAACACTAGAGCCAGTGGAAGAAGGCGGTTATTTCGTCTCGTTCCCGGATATCCCCGAAGCTTTAAC
TCAAGGGGAGACACGGGAAGAAGCGCTAGAGATGGCACTTGATGCGCTAATAACGTCTTTCGAATTTTACTTCGAAGATA
ATGAGAAAATTCCGCTTCCTAGCCCTATAGGACAAGATGACGATTATGTCGATGTGCCCTTAAGCATAACCTCTAAGGTG
CTTATGCTTAATGCGTTTATTGATTCTAAATTAACGCAAACTGAACTGGCCAGCCGTATGGGAGTGAAGAAACAGGAAGT
CACGCGCATCTTCGATTTACGGCATTCAACTAAGATAGATACAGTGGGTAAGGCGGCAGCGGTTATCGGGCACCAGTTAA
CATTGTCAATGCAGTAA