Detailed information of TA system TA142127

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 4015517..4016210
• Replicon: chromosome
• Accession: NZ_CP045554
• Organism: Pseudomonas sp. 14181154

Toxin (Protein)

• Gene name: tad
• Uniprot ID: N2IHR9
• Locus tag: GEV39_RS18570
• Protein ID: WP_003151133.1
• Coordinates: 4015833..4016210 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: N2IIN5
• Locus tag: GEV39_RS18565
• Protein ID: WP_001172026.1
• Coordinates: 4015517..4015852 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GEV39_RS18535	4011965..4012717	+	753	Protein_3620	Tn3 family transposase	-
GEV39_RS18540	4013026..4013940	-	915	WP_001736514.1	HD domain-containing protein	-
GEV39_RS18545	4013987..4014322	-	336	WP_173890779.1	DUF3391 domain-containing protein	-
GEV39_RS18550	4014440..4014820	-	381	WP_001054412.1	hypothetical protein	-
GEV39_RS18555	4014817..4015143	-	327	WP_000091614.1	hypothetical protein	-
GEV39_RS18560	4015167..4015502	-	336	WP_000741275.1	hypothetical protein	-
GEV39_RS18565	4015517..4015852	-	336	WP_001172026.1	helix-turn-helix domain-containing protein	Antitoxin
GEV39_RS18570	4015833..4016210	-	378	WP_003151133.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GEV39_RS18575	4016364..4016612	+	249	WP_012585400.1	type II toxin-antitoxin system YafQ family toxin	-
GEV39_RS18580	4016739..4017314	+	576	WP_001173919.1	recombinase family protein	-
GEV39_RS18585	4017347..4017592	-	246	Protein_3630	hypothetical protein	-
GEV39_RS18590	4017722..4018513	+	792	WP_173890780.1	dienelactone hydrolase family protein	-
GEV39_RS18595	4018689..4019390	+	702	WP_173890781.1	4'-phosphopantetheinyl transferase superfamily protein	-
GEV39_RS18600	4019394..4021001	-	1608	WP_173890782.1	HAMP domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	4008192..4017314	9122

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-109)

Antitoxin

• Cro/C1-type helix-turn-helix domain (40-93)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13705.78 Da
• Isoelectric Point: 9.4693

>T142127 WP_003151133.1 NZ_CP045554:c4016210-4015833 [Pseudomonas sp. 14181154]
MTNKEKPLEWIASSHKDLMALPSDVRRRFGYALSLAQIGDQDDAAKVLKGFGGAGVLEVVEDDAGGTYRAVYTVKFAEAV
FVLHCFQKKSKSGIATPKADMDIIRARLKVAEVLAQELRNAKTNH

Nucleotide

• Length: 378 bp

>T142127 NZ_CP060401:c3087540-3087115 [Xenorhabdus nematophila]
ATGAAAACGGTTTACATGCTGGATACCAATATTTGTTCATTCATTATGCGTGAACAACCTGTTGCAGTAATTAAACAATT
ACAGAGATGCGTGATGAATAACCATCTGATCGTTGTATCTGCGATTACTTATGCGGAAATGCGCTTCGGGGCAGTCGGAA
AGAAAGCTTCCCCGAAACATATTGTATTAGTTGATGAGTTTTGTAGACGATTAGATAAAATTTTACCTTGGGATAAATCC
GCGGTAGACGCAACAACTAAAACAAAGATTGCATTAGCTGCTGCTGGAACGCCTATTGGACATAATGATGCTGCTATTGC
AGGGCATGCTTTGTCTGTAGGAGCGACATTGATAACAAACAATAGTCGTGAATTTTCTCGTGTCACTGGACTATCATTAG
AAGATTGGGCTAACGGGCTGATTTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12201.39 Da
• Isoelectric Point: 10.6058

>AT142127 WP_001172026.1 NZ_CP045554:c4015852-4015517 [Pseudomonas sp. 14181154]
MQKRIIEGVEVQRSSGNVFADLGLPDAEKLKIKTGLVVEIRRAMRALGLTQQAAAKRMGIPQPKVSGMMRGDFTNLSERK
LMDCLNRLGYDIEIKVRPAAEPIGHLTLATA

Nucleotide

• Length: 336 bp

>AT142127 NZ_CP060401:c3087764-3087537 [Xenorhabdus nematophila]
ATGCGAACTGTAGCGATTTTTAAAAATGGTAATAATCGCGCTATCCGTTTACCTAAAGATCTGGATTTTGAAGGCGTAAA
TGAACTGGAGATTATCCGTGAAGGAGAAGCCATTATATTGCGTCCGATAAAACCCAGTTGGGATTCATTTCTTTATGAGG
ACAAAGCAGACGCAGATTTTCTGTCTGAACGCCCAGATATTGTCGAGGAAGGGCGTGTGAATTTATGA

Structures

Toxin

• AlphaFold DB: A0A024ELN5

Antitoxin

• AlphaFold DB: A0A024EKI7