Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PP_4151-4152/excise(antitoxin)
Location 3981913..3982952 Replicon chromosome
Accession NZ_CP045553
Organism Pseudomonas sp. 13159349

Toxin (Protein)


Gene name PP_4152 Uniprot ID A0A2N5BP56
Locus tag GEV38_RS18455 Protein ID WP_015271072.1
Coordinates 3982377..3982952 (+) Length 192 a.a.

Antitoxin (Protein)


Gene name PP_4151 Uniprot ID A0A2N5BP88
Locus tag GEV38_RS18450 Protein ID WP_015271071.1
Coordinates 3981913..3982380 (+) Length 156 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GEV38_RS18425 3976972..3978045 + 1074 WP_009686399.1 microcin C ABC transporter permease YejB -
GEV38_RS18430 3978047..3979066 + 1020 WP_013973404.1 ABC transporter permease -
GEV38_RS18435 3979068..3980678 + 1611 WP_054572484.1 ABC transporter ATP-binding protein -
GEV38_RS18440 3981069..3981311 + 243 WP_054572483.1 hypothetical protein -
GEV38_RS18445 3981468..3981731 + 264 WP_015271070.1 DUF3077 domain-containing protein -
GEV38_RS18450 3981913..3982380 + 468 WP_015271071.1 helix-turn-helix domain-containing protein Antitoxin
GEV38_RS18455 3982377..3982952 + 576 WP_015271072.1 PIN domain-containing protein Toxin
GEV38_RS18460 3983167..3986097 + 2931 WP_015271073.1 aminotransferase -
GEV38_RS18465 3986461..3987366 + 906 WP_013973410.1 LysR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3960880..3986097 25217


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(9-115)

Antitoxin

SinI-like, DNA-binding domain

(84-129)


Sequences

Toxin        


Download         Length: 192 a.a.        Molecular weight: 21970.23 Da        Isoelectric Point: 5.0798

>T142125 WP_015271072.1 NZ_CP045553:3982377-3982952 [Pseudomonas sp. 13159349]
MKHSPFTAIYDANVLYPAPLRDFLMNLALTGIYRARWSTKIHDEWKRNLLLNRPDLTLEQVDRTSRRMDAAVPDALVTDY
ESLVEGLDLPDKDDRHVLAAAIKCNASVIVTFNLKDFPETALDVFDIEPLHPDDFIADLWDLDKAAVLEAAQRQRMSLKN
PPHSVQQYLDRLLQQKLPESVKLLSEFKFLL

Download         Length: 576 bp

>T142125 NZ_CP060401:2949506-2949700 [Xenorhabdus nematophila]
ATAGACTACATATCTATAGATTGTAGTCTATATACTCGAACAATCCGGGCATTTTTTGCATTTGATCCAGAACGTCAGGC
TGTTGTTTTTTGCGCAGGAAATAAAGCAGGGAAAAACCAAAAGTCATTCTATCGGGATATGATTAAGGTTGCTGACGAAC
AATTCAGAATCCATCTAAACAATTTGGAGAAATGA

Antitoxin


Download         Length: 156 a.a.        Molecular weight: 17366.86 Da        Isoelectric Point: 5.3408

>AT142125 WP_015271071.1 NZ_CP045553:3981913-3982380 [Pseudomonas sp. 13159349]
MTTADLSRKMLPDEKEIAVAVESSRTLAAFLSTKSDTQRIELLNEENQRQVVEVPTFALRLFGEILSELALGNSVKVVPI
HAELTTQEAADLLNVSRPYLVKLLDESVIPHTKTGRHRRVKFSDLVAYKDKRDEVSRAAMDALAAEAQELRMGYE

Download         Length: 468 bp

>AT142125 NZ_CP060401:2949702-2950001 [Xenorhabdus nematophila]
ATGGCTACTTTAAAAGAACTGATGGCAAAACGTAGCCCAGAAAGTCAAGCCAAAATAGAAGAAAAAGCAGCCGAAATGTT
GCTTGAAACGCAACTATACCAAATTCGTGAAGCATTAGCCCTTTCACAAACTCAGGTAGCAAAAAACCTGGGTATAGCCC
AATCATCAGTTGCAGCTATTGAACAACGAGGAAATGAATTGAAAATTTCCACACTGAAAAAATACATTGAAGCGATGGGC
GGCGAAATGACTTTGGGAGTCAAATTACCTGATGGAAAATTAATGAATTTCTCCATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2N5BP56

Antitoxin

Source ID Structure
AlphaFold DB A0A2N5BP88

References