Detailed information of TA system TA142121

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 5571214..5571800
• Replicon: chromosome
• Accession: NZ_CP045552
• Organism: Pseudomonas aeruginosa strain YT12746

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: GEV42_RS26440
• Protein ID: WP_003120987.1
• Coordinates: 5571501..5571800 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: GEV42_RS26435
• Protein ID: WP_003448662.1
• Coordinates: 5571214..5571504 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GEV42_RS26415	5566365..5566574	+	210	WP_003105733.1	cold-shock protein	-
GEV42_RS26420	5566796..5568685	+	1890	WP_016851610.1	hypothetical protein	-
GEV42_RS26425	5568682..5570658	+	1977	WP_016851611.1	DEAD/DEAH box helicase	-
GEV42_RS26430	5570799..5571143	+	345	WP_016851612.1	hypothetical protein	-
GEV42_RS26435	5571214..5571504	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
GEV42_RS26440	5571501..5571800	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GEV42_RS26445	5572002..5573126	+	1125	WP_012076859.1	TcpQ domain-containing protein	-
GEV42_RS26450	5573126..5574835	+	1710	WP_012076860.1	PilN family type IVB pilus formation outer membrane protein	-
GEV42_RS26455	5574839..5576164	+	1326	WP_003099758.1	type 4b pilus protein PilO2	-
GEV42_RS26460	5576154..5576687	+	534	WP_003099760.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5543429..5633126	89697

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T142121 WP_003120987.1 NZ_CP045552:c5571800-5571501 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T142121 NZ_CP060401:c502739-502455 [Xenorhabdus nematophila]
ATGACTTATAGTCTCAAATTTGAAAAGCGGGCACTAAAAGAGTGGGAAAAACTAGGGCATCCTATCCGTGAGCAGTTCAA
AAAGAAATTGCCTGAGCGTTTAGAAAACCCCCATATATCTTCAGCTCGATTGAGTGGCCGCAATAATCTCTACAAAATCA
AATTATGTTCTTCTGGCTATCGTTTAGTCTACGAAGTCAATGATCATGCTATTGTTTTACTGGTGATCGCTGTTGGTAAA
AGATCTGGAAATCAGGTTTATTCAGTCGCTGATGAACGTGAATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT142121 WP_003448662.1 NZ_CP045552:c5571504-5571214 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT142121 NZ_CP060401:c502980-502729 [Xenorhabdus nematophila]
ATGTCTTATCAGATCCTGACAACAATAGCAGCCAGCATTACCGATTTGAAACGCAATCCCATGGGAACCGTCGCTGATGG
TGAAGGTGGTGCTGTCGCTATCCTGAACCGTAATGAACCTGCATTCTATTGTGTTCCACCGGAGCTTTACGCTTACTATT
TAGAGCTTGCAGAGGATGCAGAATTAAATCACATCGCAGATGAACGGATGAAATCCGCTGAATTTGTGAGCGTCGATCTC
AATGACTTATAG

Structures

Toxin

• AlphaFold DB: G8CP73