Detailed information of TA system TA142117

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB(antitoxin)
• Location: 2568224..2569018
• Replicon: chromosome
• Accession: NZ_CP045552
• Organism: Pseudomonas aeruginosa strain YT12746

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A844NUK8
• Locus tag: GEV42_RS12135
• Protein ID: WP_021263385.1
• Coordinates: 2568839..2569018 (-)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: GEV42_RS12130
• Protein ID: WP_021263386.1
• Coordinates: 2568224..2568799 (-)
• Length: 192 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GEV42_RS12095	2563482..2564054	-	573	WP_023088260.1	S24 family peptidase	-
GEV42_RS12100	2564432..2564650	+	219	WP_016561979.1	helix-turn-helix domain-containing protein	-
GEV42_RS12105	2564682..2565254	+	573	WP_019682191.1	hypothetical protein	-
GEV42_RS12110	2565257..2565727	+	471	Protein_2393	hypothetical protein	-
GEV42_RS12115	2566191..2566799	+	609	WP_021263389.1	hypothetical protein	-
GEV42_RS12120	2566792..2567235	+	444	WP_021263388.1	RusA family crossover junction endodeoxyribonuclease	-
GEV42_RS12125	2567264..2568133	+	870	WP_021263387.1	hypothetical protein	-
GEV42_RS12130	2568224..2568799	-	576	WP_021263386.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
GEV42_RS12135	2568839..2569018	-	180	WP_021263385.1	type II toxin-antitoxin system HicA family toxin	Toxin
GEV42_RS12140	2569107..2569322	+	216	WP_019486654.1	hypothetical protein	-
GEV42_RS12145	2569465..2569854	+	390	WP_004349441.1	hypothetical protein	-
GEV42_RS12150	2569847..2570131	+	285	WP_016852757.1	phage holin family protein	-
GEV42_RS12155	2570140..2570736	+	597	WP_021263384.1	terminase small subunit	-
GEV42_RS12160	2570723..2572018	+	1296	WP_021263383.1	terminase family protein	-
GEV42_RS12165	2572021..2573376	+	1356	WP_021263382.1	DUF4055 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2537534..2597196	59662

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6591.62 Da
• Isoelectric Point: 11.1475

>T142117 WP_021263385.1 NZ_CP045552:c2569018-2568839 [Pseudomonas aeruginosa]
MKFSEFRRWLRAQGVSFEAGKGSHFKVTAPNGKTTTFADHGAKEMPEPTRKAIIKQLGL

Nucleotide

• Length: 180 bp

>T142117 NZ_CP060399:c4776306-4776154 [Xanthomonas hortorum pv. vitians]
ATGAAGCGACTGCTGACACTGATGGTGCTAGGCCTGTTTTCGGCTGGCGTGATGACTGGCTGCAACACCATGGCCGGCGC
CGGCAAGGACATGCAGGGCGCAGGTGAGAAGGTCGAAAAGAAGGCCGATAATTGCAGCGACGGCAAGTGCTGA

Antitoxin

Protein

• Length: 192 a.a.
• Molecular weight: 21294.78 Da
• Isoelectric Point: 8.3556

>AT142117 WP_021263386.1 NZ_CP045552:c2568799-2568224 [Pseudomonas aeruginosa]
MYNFAIRFEQDDSAPGVAVFCRDLPELNSYGDTVEHALSEAVDAIETTLSLYVDQRRSIPVASAPEPEEHVVHLPAVTVA
KIALWNEMMRRGMRKADLCRVLGIAQVQGDRLVDFLHTSKMEQVESALAALEMRLVICAEPVKQPKQCKGAGCIRDRRLV
KARRLLRARLLELTESSGAFTRAPKIKKPGI

Nucleotide

• Length: 576 bp

>AT142117 NZ_CP060399:c4776075-4775941 [Xanthomonas hortorum pv. vitians]
ATGAAGCGTGCAATTATACTGCTGGTGCTGTCGGTGTTTTCGGTCGGTATGCTGGCGGGTTGCAACACCGTCGCAGGTGC
TGGCAAGGACGTGCAGGGCGCTGGCGAGAAAGTGGAAGACGCTGCGCGCAAGTGA

Structures

Toxin

• AlphaFold DB: A0A844NUK8