Detailed information of TA system TA142113

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 2047171..2048210
• Replicon: chromosome
• Accession: NZ_CP045551
• Organism: Pseudomonas putida strain 15420352

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: GEV41_RS09245
• Protein ID: WP_098091090.1
• Coordinates: 2047171..2047746 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: A0A173HI04
• Locus tag: GEV41_RS09250
• Protein ID: WP_003251469.1
• Coordinates: 2047743..2048210 (-)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GEV41_RS09235	2042788..2044023	+	1236	WP_003251472.1	FAD-dependent oxidoreductase	-
GEV41_RS09240	2044027..2046957	-	2931	WP_173879715.1	aminotransferase	-
GEV41_RS09245	2047171..2047746	-	576	WP_098091090.1	PIN domain-containing protein	Toxin
GEV41_RS09250	2047743..2048210	-	468	WP_003251469.1	helix-turn-helix domain-containing protein	Antitoxin
GEV41_RS09255	2048355..2049962	-	1608	WP_173879718.1	ABC transporter ATP-binding protein	-
GEV41_RS09260	2049964..2050983	-	1020	WP_003251467.1	ABC transporter permease	-
GEV41_RS09265	2050985..2052058	-	1074	WP_003251466.1	microcin C ABC transporter permease YejB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-115)

Antitoxin

• Helix-turn-helix domain, group 17 (84-132)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21697.95 Da
• Isoelectric Point: 5.5710

>T142113 WP_098091090.1 NZ_CP045551:c2047746-2047171 [Pseudomonas putida]
MKHSPFTAIYDANVLYPAPLRDFLMNLALTGIYRARWSASIHDEWKRNLLLNRPDLTPEHLDRTSSLMDAAVPDALVTDY
DSLVEGLDLPDRDDRHVLAAAIKCNASVIVTFNLKDFPKAILGAFDIEPLHPDDFIADLWDLDKAAVLEAAQRQRISLKN
PPHNVQQYLDRLLQQKLPETVKLLSAFKFLL

Nucleotide

• Length: 576 bp

>T142113 NZ_CP060399:c2702251-2702045 [Xanthomonas hortorum pv. vitians]
ATGCAAGCTGTAGCCGAACGGAAGCTGCAATTGCTGGATTCGGCGCAGACGCTGGAGTTCTTGCGCAGCCCGCCGGGCAA
TCGGTTTGAGCTTCTTGCGGGTACGCGTGCAGGTCAGCACAGCATCCGCATCAATGACCAATGGCGTGTGTGCTTTGTCT
GGACTGACGCCGGCCCCGAGCACGTTGAGATTGTCGATTACCACTGA

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17229.71 Da
• Isoelectric Point: 6.6505

>AT142113 WP_003251469.1 NZ_CP045551:c2048210-2047743 [Pseudomonas putida]
MTTANLSRHVLPDEKEIAVAVESSRTLAAFLSTKSDTQRIELLNEENQRQVVEVPTFALRLFGEILSELALGNSVKVVPI
HAELTTQEAADLLNVSRPHLVKLLDEAVIPHTKTGRHRRVKFSDLVAYKDKRDGVSRTAMDALAAQAQELKMGYE

Nucleotide

• Length: 468 bp

>AT142113 NZ_CP060399:c2702035-2701736 [Xanthomonas hortorum pv. vitians]
ATGCGACCCAACAACAAACTGCGCGCCATCCATCCCGGCGAAATCCTGCGGGAAGATTTCATGCTGCCGCTGGGGCTGAC
CATTAACGGCCTGGCGCGGGCGCTGGATGTACCGGCAACGCGCATCCACAGCATCGTGCATGGCGAGCGTGCCATCAGCG
CCGATACCGCAGCGCGGCTTGCACGCCACTTCGGTGGCGATGCGGCGTCCTGGCTCGCGCTGCAGGCCACGTATGAACTC
AAGACCTTGCCCAACCGCAGCGAGATCGAGCGCAACGTGCAGCCGCGTGTGGCGGCCTAG

Structures

Antitoxin

• AlphaFold DB: A0A173HI04