Detailed information of TA system TA142111

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 985420..986006
• Replicon: chromosome
• Accession: NZ_CP045551
• Organism: Pseudomonas putida strain 15420352

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: GEV41_RS04430
• Protein ID: WP_173881370.1
• Coordinates: 985420..985713 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: I3UY28
• Locus tag: GEV41_RS04435
• Protein ID: WP_004576347.1
• Coordinates: 985710..986006 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GEV41_RS04405	981021..981875	+	855	WP_173879440.1	putative selenate ABC transporter substrate-binding protein	-
GEV41_RS04410	981872..982681	+	810	WP_003248576.1	phosphonate ABC transporter ATP-binding protein	-
GEV41_RS04415	982675..983502	+	828	WP_003248575.1	ABC transporter permease subunit	-
GEV41_RS04420	983499..984266	+	768	WP_003248573.1	phosphonate ABC transporter, permease protein PhnE	-
GEV41_RS04425	984303..985196	-	894	WP_003248571.1	hypothetical protein	-
GEV41_RS04430	985420..985713	+	294	WP_173881370.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GEV41_RS04435	985710..986006	+	297	WP_004576347.1	putative addiction module antidote protein	Antitoxin
GEV41_RS04440	986017..986496	-	480	WP_102885856.1	GNAT family N-acetyltransferase	-
GEV41_RS04445	986666..987313	-	648	WP_054889318.1	serine protease	-
GEV41_RS04455	987766..988815	+	1050	WP_004576301.1	tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA	-
GEV41_RS04460	988830..989945	+	1116	WP_003248558.1	tRNA guanosine(34) transglycosylase Tgt	-
GEV41_RS04465	989988..990323	+	336	WP_003248556.1	preprotein translocase subunit YajC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (2-96)

Antitoxin

• Uncharacterized protein HI1420 (4-93)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10779.65 Da
• Isoelectric Point: 10.9252

>T142111 WP_173881370.1 NZ_CP045551:985420-985713 [Pseudomonas putida]
MFLVEQTESFAAWLSSLRDLRARLAIGRRLERAAAGNLGDFKWLGGGIGELRIDVAAGYRVYFTQKGQRLVLLLVGGDKS
TQAAEILKARKLLKEMK

Nucleotide

• Length: 294 bp

>T142111 NZ_CP060398:2044648-2045133 [Synechococcus sp. CBW1002]
ATGCGCCCGCCTGAGCCCCTCGGCCCCCAGCATCAGCTGGAAGGCTTCGACTGCGGCAACACCGCATTGAACCGCTGGCT
GCAGCAGCGGGCCCTGGCCAATGAGCGCCAAGGGGTGTCGCGCACCGTGGTGGTCTGCTCAGACGAGGACAGCAACGTGA
TCGCCTACGCCTGCCTGTCGGCTGGCGCAATCGTGCTGGCAGAGGTGCCTGGTGCCTTGCGACGCAACATGCCGGACCCG
CTTCCCGTTGTAGTGCTGGGCCGCCTGGCGGTTGACCGACGCCATCAAGGCCAGGGCCTCGGCCGCGCCCTGGTGGCCGA
TGCGCTGCGGCGCAGCCTGGCCGCTCGGCGCCTGATTGGCGCCAGAGCCCTGCTCGTGCACGCGATCGATGAGCAGGCGG
CTGCGTTCTACCGCTCGCTTGGCTTTCGCCCCTCGCCGCTCAGCGCCTCGACGTTAATGCTGCAGCTCTCCGAACCAGAC
GGCTGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10676.33 Da
• Isoelectric Point: 6.7249

>AT142111 WP_004576347.1 NZ_CP045551:985710-986006 [Pseudomonas putida]
MSEKLIPFDMAALLSSDEAISEYLSQVLSNGDTEEIIRALGHIARARGMTHIATESGLGRESLYKALSPGAKPRFDTVLR
VIRALGVDLLVQPHAMTR

Nucleotide

• Length: 297 bp

>AT142111 NZ_CP060398:2044380-2044661 [Synechococcus sp. CBW1002]
ATGGCGCGCGATCAGGTGCTGCAGCTGCGGGCCTCGGCTGAGCAGCGCCAGCTGATTGATCAGGCCGCTGCGGCGGAAGG
CATCAGCCGCACCGAATTCATCCTGCGCAGCTGCCAGGAACGGGCACGCGATGTGCTCCTGGATCGCACACTGTTCTCCC
TCACGCAGGAGCAGACCACGGCCCTGCTGGCTGATCTGGAGGATTCCGGCGCTGCCGCCCAGGATCAGGCCAGCATCGAG
CGCCTGCTCGACCTGCCGCAGCCGTGGCATGCGCCCGCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A2I0CD26