Detailed information of TA system TA142106

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 37459..38036
• Replicon: plasmid pIB_NDM_1
• Accession: NZ_CP045540
• Organism: Proteus mirabilis strain CRE14IB

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: U5N651
• Locus tag: GD587_RS18835
• Protein ID: WP_023159957.1
• Coordinates: 37704..38036 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: GD587_RS18830
• Protein ID: WP_096864992.1
• Coordinates: 37459..37704 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GD587_RS18795	33212..34258	+	1047	WP_072070749.1	ParB/RepB/Spo0J family partition protein	-
GD587_RS18800	34261..34605	+	345	WP_023159966.1	single-stranded DNA-binding protein	-
GD587_RS18805	34598..35020	+	423	WP_048821775.1	hypothetical protein	-
GD587_RS18810	35030..35422	+	393	WP_109910891.1	hypothetical protein	-
GD587_RS18815	35772..36140	+	369	WP_109910890.1	hypothetical protein	-
GD587_RS18820	36238..36465	-	228	WP_096864993.1	DNA partition complex ParG	-
GD587_RS18825	36566..37189	-	624	WP_109910889.1	AAA family ATPase	-
GD587_RS18830	37459..37704	+	246	WP_096864992.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
GD587_RS18835	37704..38036	+	333	WP_023159957.1	endoribonuclease MazF	Toxin
GD587_RS18840	38067..38447	+	381	WP_096864991.1	hypothetical protein	-
GD587_RS18845	38533..38676	-	144	WP_071547955.1	type I toxin-antitoxin system Hok family toxin	-
GD587_RS18850	39041..39838	-	798	WP_048607643.1	hypothetical protein	-
GD587_RS18855	40409..41197	+	789	WP_163939961.1	site-specific integrase	-
GD587_RS18860	41211..42050	+	840	WP_094962566.1	DUF4365 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	dfrA14 / ARR-3 / blaOXA-10 / ant(3'')-Ia / sul1 / aph(3')-VI / blaNDM-1 / tet(B)	-	1..99278	99278

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (9-107)

Antitoxin

• SpoVT-AbrB domain (7-49)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12097.88 Da
• Isoelectric Point: 7.0813

>T142106 WP_023159957.1 NZ_CP045540:37704-38036 [Proteus mirabilis]
MVSRYVPDSGDLIWIDFDPVEGHEQGGHRPAVVLSPFAYNNKVGLLLCVPCTTKGKGYPFECELSNERDSYALADQVTCV
DWRARKVTKKGKVEASELAEIKAKAKALIG

Nucleotide

• Length: 333 bp

>T142106 NZ_CP060398:c1212815-1212558 [Synechococcus sp. CBW1002]
GTGCTGGGTGTAGTCGTGGTGCGAGCGAAGCTGGTCAAAATCGGCAACTCGAGAGGGATCCGCCTCGCCAAGCCACTGCT
TGAGGTTGCGGGGCTCGCTGACGAAGTCGAGATCGAAGCGGCTCCAGGGGTTCTGACCATCAGGCCATCCGCTCATCCGA
GGGCTGGCTGGGCAGAGGCGGCATCGTCTTTGGAGCCAGAGGGACTTCTGGATGAAATGAGCGCCACCCAGTTTGATGAC
GAAGAGTGGTCATGGTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8800.23 Da
• Isoelectric Point: 5.0508

>AT142106 WP_096864992.1 NZ_CP045540:37459-37704 [Proteus mirabilis]
MAQVTVKKWGNSPSVRLPVAIMQKAALSVDDTVEIAVEEGRIIITPVKAVEYSLENLLAGITPENIHEKVDFGARTGKEL
I

Nucleotide

• Length: 246 bp

>AT142106 NZ_CP060398:c1213259-1212951 [Synechococcus sp. CBW1002]
ATGACAAAAACAATCACGACTATCTGGGACCCGGCCGCACACCTAACCACTGCCGATGATGTCGCGGCTTATTTGGAAGC
TGCGCTTCAAGATGGAGATCCTCAGCTTGTAGCCGCTGCTCTTGGCGATATTGCTAGGGCCAAAGGGATGAGCCAAATCG
CACGTGAGGCGGGTCTAGGCAGGGAGAGTCTGTACAAGTCCCTATCCTCTTCAGGAAATCCAGAATTAGCCACGGTATTG
AAGGTTATCTCGGCACTAGGCTTGCAACTACATGTCAGCGCTACTGACAATGCGAAAATGGCTGTCTAA

Structures

Toxin

• AlphaFold DB: A0A2P1BQ75