Detailed information of TA system TA142104

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 3245524..3246545
• Replicon: chromosome
• Accession: NZ_CP045538
• Organism: Proteus mirabilis strain CRE14IB

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: GD587_RS15215
• Protein ID: WP_152964354.1
• Coordinates: 3245976..3246545 (+)
• Length: 190 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: B4F2H3
• Locus tag: GD587_RS15210
• Protein ID: WP_004245528.1
• Coordinates: 3245524..3245976 (+)
• Length: 151 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GD587_RS15190	3241310..3241840	-	531	WP_004245524.1	RNA pyrophosphohydrolase	-
GD587_RS15195	3242109..3243122	+	1014	WP_112843010.1	thiamine ABC transporter substrate binding subunit	-
GD587_RS15200	3243122..3244702	+	1581	WP_046334723.1	thiamine/thiamine pyrophosphate ABC transporter permease ThiP	-
GD587_RS15205	3244695..3245396	+	702	WP_004245527.1	thiamine ABC transporter ATP-binding protein ThiQ	-
GD587_RS15210	3245524..3245976	+	453	WP_004245528.1	helix-turn-helix domain-containing protein	Antitoxin
GD587_RS15215	3245976..3246545	+	570	WP_152964354.1	PIN domain-containing protein	Toxin
GD587_RS15220	3246661..3249015	+	2355	WP_152964355.1	DNA polymerase II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-113)

Antitoxin

• SinI-like, DNA-binding domain (80-125)

Sequences

Toxin

Protein

• Length: 190 a.a.
• Molecular weight: 21600.10 Da
• Isoelectric Point: 7.3470

>T142104 WP_152964354.1 NZ_CP045538:3245976-3246545 [Proteus mirabilis]
MTNYTVLLDACVMYPAPLRSYLMYLANTGLFRARWSNQIHDEWITNLLVNNPTLTAERLIRTRNLMNAHVPDCLVEGYEP
LICGLKLPDPNDRHVLAAAIKGQAQAIITFNLKDFPQKYLNSFDISAIHPDEFLSDMFELNPSSCIKAAQQHRQSLKDPP
MTVETFLGCLQKQKLPQFISHLRAFQILL

Nucleotide

• Length: 570 bp

>T142104 NZ_CP060397:3028032-3028310 [Synechococcus sp. CBW1004]
ATGATCCGGTCGTTCCGATGCGCCGACACCGAAACACTGGCCCAGGGTTGGGAGGTACCGCGCTTCCAGGCGTTCGAGCG
GGTGGCGCGCCGCAAGCTGCGGCAACTGGAGATCGCCCAACGCCTGGAGGATCTGCGCGTGCCGCCTGGCAACCGTCTCG
AACTGCTGCGGGGAGATCGGGCCGGGCAGCACAGCATCCGCATCAATGACCAGTTCCGGCTCTGCTTTCGCTGGAGTGAC
AGCGGTCCGGAAGACGTTGAGATCGTCGACTACCACTGA

Antitoxin

Protein

• Length: 151 a.a.
• Molecular weight: 16700.15 Da
• Isoelectric Point: 5.7723

>AT142104 WP_004245528.1 NZ_CP045538:3245524-3245976 [Proteus mirabilis]
MNTISERLSAEDIALAKLSSQELSAVMDINGKTQKLSIIDKTGNNHIVQIPNSALRLMIEILTQLGQGNTVSITPIHAEL
TTQEAADILNMSRPTFVKLLDTRAIPYSRKGNRRKVAYADIVAYKNELEQKRLDALAELSQLDQEMDMGY

Nucleotide

• Length: 453 bp

>AT142104 NZ_CP060397:3028320-3028613 [Synechococcus sp. CBW1004]
ATGGACCGCCTCACTCCCATCACCCCCGGTGAACTGCTCCTGCAGGAGTTCCTCGAACCGTTGGGCATCAGTCAGTACCG
CCTGGCCAAGGCCATCGACGTGCCCGCCTCCCGCATCAGCGAGATCATCACCGGACAACGAGCGATCACCGCCGACACCG
ATCTGCGCCTCTGCCGCGTGCTGGGGCTCAGTCCGGGGTATTGGTTGCGTGCGCAGGCTGCCCACGACACGGAGGTCGCC
AGTGCTGAGCTGGCGGAAGAGCTGGAACGGATCCAGCCGTTGGTGGGTTGCTGA

Structures

Antitoxin

• AlphaFold DB: B4F2H3