Detailed information of TA system TA142092

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 4165001..4165838
• Replicon: chromosome
• Accession: NZ_CP045526
• Organism: Shigella sonnei strain 6607.69

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: GE169_RS21205
• Protein ID: WP_000227784.1
• Coordinates: 4165001..4165543 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: GE169_RS21210
• Protein ID: WP_001297137.1
• Coordinates: 4165527..4165838 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GE169_RS21175 (4160066)	4160066..4160557	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
GE169_RS21180 (4160685)	4160685..4162049	-	1365	WP_001000971.1	MFS transporter	-
GE169_RS21185 (4162168)	4162168..4162865	+	698	WP_237158184.1	IS1-like element IS1A family transposase	-
GE169_RS21190 (4163233)	4163233..4163631	+	399	Protein_4134	SEL1-like repeat protein	-
GE169_RS21200 (4164379)	4164379..4164945	+	567	Protein_4136	SEL1-like repeat protein	-
GE169_RS21205 (4165001)	4165001..4165543	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
GE169_RS21210 (4165527)	4165527..4165838	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
GE169_RS21215 (4166023)	4166023..4166913	-	891	WP_000971327.1	heme o synthase	-
GE169_RS21220 (4166925)	4166925..4167254	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
GE169_RS21225 (4167254)	4167254..4167868	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
GE169_RS21230 (4167858)	4167858..4169849	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
GE169_RS21235 (4169871)	4169871..4170818	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T142092 WP_000227784.1 NZ_CP045526:c4165543-4165001 [Shigella sonnei]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T142092 NZ_CP060396:c3209802-3209302 [Synechococcus sp. CBW1006]
ATGGAGCTGCTGGTGCCGCAGCCCCTGGCCAGCCACCACGGCACCGCCGGCTTCGACTGCGGCGACACCAGCCTGGATCA
CTGGCTCCGGCAGCGTGCCCTGCCGAATCAGCAGAGCGGTGCCACACGCACGTTTGTGGTCTGCGATGGCGACGGCACCG
TCAAGGCCTATGGGGCTCTGGCCTCCGGTGCCGTGGCGGTGGCCTCCGCACCCGGGAGCTTTCCTCGCAATAGGCCCGAT
CCCATCCCGGTTGTACTGCTGGCACGGCTGGCGGTCTGCCGCGGTGTGCAAGGTCGCGGCATCGCGCGTGCCCTGCTTGC
CGATGCGTTCGAGCGGGTCCTTCAGGCCAGCGAACGGATCGGCGTTCGCGGCATCGTGGTCCACGCTGCTTCCGCGGACG
CCCGTAACTTCTATCTGCACATGGGTTTCGATCCCTCACCCATCGATCCCGACACCCTCCTGATCCGTCTCTCGGATGTG
GCGGCAACCCTGGGGTTGTGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT142092 WP_001297137.1 NZ_CP045526:c4165838-4165527 [Shigella sonnei]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT142092 NZ_CP060396:c3210074-3209802 [Synechococcus sp. CBW1006]
GTGGCTGCCACCCCTCGCCTCAGCTGGAACCTGCGGGTCTCGCCCGACGATCAGGCCCTGATCGATCGCGCCGTCCGTGC
CTCCGGGCTCACTCGAACCGACTTCGTTCTGCAGGCGGCCCGCACCGCCGCCCGTGAGCTTCTGGTCCAGCAGAGCTGGC
AGTCCGTGTCGTCCGAACATTTTGACCAGCTTCTCCGCCTGCTGGATGCTCCCCCAGGCCCCAGTGACCGCCTGCAACGC
ACCATGACCGCGCCGAGGCCCTGGCAACGCTGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3