Detailed information of TA system TA142066

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 2248163..2249000
• Replicon: chromosome
• Accession: NZ_CP045524
• Organism: Shigella sonnei strain 6904.27

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: GE168_RS11500
• Protein ID: WP_000227784.1
• Coordinates: 2248458..2249000 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: GE168_RS11495
• Protein ID: WP_001297137.1
• Coordinates: 2248163..2248474 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GE168_RS11470 (2243183)	2243183..2244130	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
GE168_RS11475 (2244152)	2244152..2246143	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
GE168_RS11480 (2246133)	2246133..2246747	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
GE168_RS11485 (2246747)	2246747..2247076	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
GE168_RS11490 (2247088)	2247088..2247978	+	891	WP_000971327.1	heme o synthase	-
GE168_RS11495 (2248163)	2248163..2248474	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
GE168_RS11500 (2248458)	2248458..2249000	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
GE168_RS11505 (2249056)	2249056..2249622	-	567	Protein_2251	SEL1-like repeat protein	-
GE168_RS11510 (2249656)	2249656..2250353	+	698	WP_237158184.1	IS1-like element IS1A family transposase	-
GE168_RS11515 (2250370)	2250370..2250768	-	399	Protein_2253	SEL1-like repeat protein	-
GE168_RS11525 (2251953)	2251953..2253317	+	1365	WP_001000971.1	MFS transporter	-
GE168_RS11530 (2253445)	2253445..2253936	-	492	WP_001138904.1	nucleotide binding protein YajQ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T142066 WP_000227784.1 NZ_CP045524:2248458-2249000 [Shigella sonnei]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T142066 NZ_CP060395:c411634-411254 [Synechococcus sp. CBW1108]
GTGATCTATCTGAGCAAGAACCGTCCGCCTCAGGTAGCCCAGCGCATCGATGCCCTCGGCGATGACGACTCCATTGCCAT
GTCGTTCATCACCTGGGCCGAGCTGCTGCGCGGCGCAGAGGGCAGCCAGCGGCGGGAGGCCACCCTGCAGCATCTCGAAG
CCCTGAGCCGGCTGGCGCCGGTGCTCTATCCACAGGGACCGGGGATTTGCGAGCACTACGCCGTTCAGGCCACCGCACTG
AAGCGCCGTGCCACACCGATCGACGCCAACGACCTCTGGATCGCTTGTCATGCTCTCGCCATCGGCGCCACTGTGGTGAG
CCACAACGTCAGTGAGTTCAGCCGGGTGGAGGGGCTGCAGCTGGTGGACTGGGCCGTCTGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT142066 WP_001297137.1 NZ_CP045524:2248163-2248474 [Shigella sonnei]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT142066 NZ_CP060395:c411885-411631 [Synechococcus sp. CBW1108]
ATGGATGTGCTGCCCAGCCGCGTGTTTATGAACGGCAACAGCCAGGCTGTGCGCATCCCCGCCGAGTTTCGCCTCAGCAG
CGATCGGGTGCAGATCAGCCGCACCCCAGAAGGCGATCTGCTCATTCACCCCTGTCCAAGCCAGCGGGGCCAGGCCCTGC
TGCAGGCCCTCAGCAGCTTCGATGCTGACTTTGTGGAGGCACTGGAGCAAGAGCAGCAAAACAAGCTGCCGGTGCAGGAA
CGGGAAGCCCTGTGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3