Detailed information of TA system TA142056

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 1025246..1025730
• Replicon: chromosome
• Accession: NZ_CP045524
• Organism: Shigella sonnei strain 6904.27

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A377EAU1
• Locus tag: GE168_RS05270
• Protein ID: WP_014334020.1
• Coordinates: 1025440..1025730 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: A0A376K4R9
• Locus tag: GE168_RS05265
• Protein ID: WP_005142544.1
• Coordinates: 1025246..1025440 (+)
• Length: 65 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GE168_RS05225 (1020441)	1020441..1020797	-	357	WP_005140877.1	hypothetical protein	-
GE168_RS05230 (1020855)	1020855..1021277	-	423	WP_001151171.1	DUF977 family protein	-
GE168_RS05235 (1021293)	1021293..1022018	-	726	WP_000450631.1	DUF1627 domain-containing protein	-
GE168_RS05240 (1022040)	1022040..1022786	-	747	WP_000788740.1	ATP-binding protein	-
GE168_RS05245 (1022793)	1022793..1023857	-	1065	WP_001262380.1	hypothetical protein	-
GE168_RS05250 (1023929)	1023929..1024354	-	426	WP_000693826.1	toxin YdaT family protein	-
GE168_RS05255 (1024351)	1024351..1024614	-	264	WP_001048456.1	helix-turn-helix transcriptional regulator	-
GE168_RS05260 (1024741)	1024741..1025226	+	486	WP_053002468.1	helix-turn-helix domain-containing protein	-
GE168_RS05265 (1025246)	1025246..1025440	+	195	WP_005142544.1	hypothetical protein	Antitoxin
GE168_RS05270 (1025440)	1025440..1025730	+	291	WP_014334020.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GE168_RS05275 (1026010)	1026010..1026162	+	153	WP_000379558.1	DUF1391 family protein	-
GE168_RS05280 (1026174)	1026174..1026539	+	366	WP_024174355.1	hypothetical protein	-
GE168_RS05290 (1027917)	1027917..1028705	+	789	Protein_1035	Rha family transcriptional regulator	-
GE168_RS05300 (1029454)	1029454..1030530	+	1077	Protein_1037	molybdopterin-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	vgrG/tssI / rhs/PAAR / rhs/PAAR / sodB	848929..1119484	270555

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11062.93 Da
• Isoelectric Point: 10.2013

>T142056 WP_014334020.1 NZ_CP045524:1025440-1025730 [Shigella sonnei]
MLPVLWLPSARDDLRQIVAYIAKENPAAARRMKIRIETSVLPLTEHPYLYPPSERVLGLREIVTHPNYIILYRVTASSIE
VVNIVHSRRQYPGKNS

Nucleotide

• Length: 291 bp

>T142056 NZ_CP060392:c183922-183641 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCCCTGAAAGCCGTCCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCTTTTCGGGTAAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTAA

Antitoxin

Protein

• Length: 65 a.a.
• Molecular weight: 7097.98 Da
• Isoelectric Point: 4.1759

>AT142056 WP_005142544.1 NZ_CP045524:1025246-1025440 [Shigella sonnei]
MSTVLSPMVSEFETVEQENSYNEWLRAKVAASLADPRPAIPHDEVMAEMEQIIAQIAAATHRGE

Nucleotide

• Length: 195 bp

>AT142056 NZ_CP060392:c184146-183919 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGATGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCGTATCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Toxin

• AlphaFold DB: A0A377EAU1

Antitoxin

• AlphaFold DB: A0A376K4R9