Detailed information of TA system TA142054

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 120405..120952
• Replicon: plasmid p5908-2
• Accession: NZ_CP045523
• Organism: Shigella flexneri strain 5908.2

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: GE167_RS23635
• Protein ID: WP_244442846.1
• Coordinates: 120674..120952 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: E1QMW3
• Locus tag: GE167_RS23630
• Protein ID: WP_000079941.1
• Coordinates: 120405..120674 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GE167_RS23605 (116051)	116051..116581	-	531	WP_161550650.1	transcription termination/antitermination NusG family protein	-
GE167_RS23610 (117028)	117028..117309	-	282	Protein_132	conjugal transfer protein TraA	-
GE167_RS24575 (117347)	117347..117505	-	159	Protein_133	ash family protein	-
GE167_RS23620 (118463)	118463..119495	+	1033	Protein_134	replication initiation protein	-
GE167_RS23630 (120405)	120405..120674	+	270	WP_000079941.1	type II toxin-antitoxin system antitoxin YacA	Antitoxin
GE167_RS23635 (120674)	120674..120952	+	279	WP_244442846.1	type II toxin-antitoxin system toxin YacB	Toxin
GE167_RS23640 (120992)	120992..121840	+	849	WP_001057996.1	3'-5' exonuclease	-
GE167_RS23645 (121957)	121957..122430	+	474	WP_001334658.1	hypothetical protein	-
GE167_RS24010 (122908)	122908..123174	-	267	WP_173020741.1	hypothetical protein	-
GE167_RS24580 (123174)	123174..123666	-	493	Protein_140	hypothetical protein	-
GE167_RS23655 (123721)	123721..124314	-	594	WP_161550651.1	ProQ/FINO family protein	-
GE167_RS23660 (124685)	124685..125389	-	705	WP_001067858.1	IS6-like element IS26 family transposase	-
GE167_RS23665 (125455)	125455..125739	+	285	Protein_143	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA1 / ant(3'')-Ia / qacE / sul2 / qnrS1 / blaCTX-M-15	-	1..137368	137368

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10761.43 Da
• Isoelectric Point: 9.7617

>T142054 WP_244442846.1 NZ_CP045523:120674-120952 [Shigella flexneri]
MEIFWTMLASQDRKRIRKYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP

Nucleotide

• Length: 279 bp

>T142054 NZ_CP060391:2520-2810 [Psychrobacter sp. KCTC 72983]
ATGGATGTTGTGAACTACACTGATTTTAGAAAGGGCCTAGCCAAATATATCGATAAGGTAGATACCGACAAATCGCCGAT
CGTGATTACTAGACAAAACGCCAAACCTGCGGTACTCATGAGTTTAGAAGAGTTTAATTCATATGAGGAAACGCTTCATC
TGCTAAAAAGCCCCACTAATGCCAAGCGCCTCAGACGCTCTATAGCCGATGCTAAGGCGGGTCGTTTAATCGAGCATGAC
ATCAATACGGATGACGCATTATTGACTTGTGATGATGAGGTGGACGCTTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10252.58 Da
• Isoelectric Point: 7.2057

>AT142054 WP_000079941.1 NZ_CP045523:120405-120674 [Shigella flexneri]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT142054 NZ_CP060391:2807-3061 [Psychrobacter sp. KCTC 72983]
TTGATCTTATCTTGGACACAGGATGCGTGGGAGGACTACGAATACTGGCAAAAAACCAGCAAAGAGAAGGTCAAGCAAAT
AAGTAAGCTGATTAAAGCCATCAAACGTGACCCATTTGAGGGTATTGGTAAACCTGAACCACTCAAACACGATCTGGCAG
GCTATTGGTCGCGTCGCATTGATCAAGAGCATAGACTGGTTTATGAGGTTCAAGATGATGCCATTATCATCGTTCAGTGC
CGATATCATTATTAA

Structures

Antitoxin

• AlphaFold DB: A0A629TXC5