Detailed information of TA system TA142049

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 17112..17653
• Replicon: plasmid p5908-2
• Accession: NZ_CP045523
• Organism: Shigella flexneri strain 5908.2

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: GE167_RS23060
• Protein ID: WP_254000279.1
• Coordinates: 17381..17653 (+)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: E1QMW3
• Locus tag: GE167_RS23055
• Protein ID: WP_000079941.1
• Coordinates: 17112..17381 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GE167_RS23030 (12754)	12754..13287	-	534	WP_001372168.1	transcription termination/antitermination NusG family protein	-
GE167_RS23035 (13729)	13729..14016	-	288	WP_000074855.1	conjugal transfer protein TraA	-
GE167_RS23040 (13934)	13934..14159	-	226	Protein_20	ash family protein	-
GE167_RS23045 (15171)	15171..16202	+	1032	WP_000907869.1	plasmid replication initiator RepA	-
GE167_RS23055 (17112)	17112..17381	+	270	WP_000079941.1	type II toxin-antitoxin system antitoxin YacA	Antitoxin
GE167_RS23060 (17381)	17381..17653	+	273	WP_254000279.1	type II toxin-antitoxin system toxin YacB	Toxin
GE167_RS23065 (17693)	17693..18541	+	849	WP_001057996.1	3'-5' exonuclease	-
GE167_RS23070 (18749)	18749..19132	+	384	WP_001372421.1	hypothetical protein	-
GE167_RS23985 (19609)	19609..19875	-	267	WP_000955263.1	hypothetical protein	-
GE167_RS23075 (19875)	19875..20369	-	495	WP_001459505.1	hypothetical protein	-
GE167_RS23080 (20425)	20425..21027	-	603	WP_000517695.1	ProQ/FINO family protein	-
GE167_RS23085 (21398)	21398..22102	-	705	WP_001067858.1	IS6-like element IS26 family transposase	-
GE167_RS23090 (22169)	22169..22453	+	285	Protein_30	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA1 / ant(3'')-Ia / qacE / sul2 / qnrS1 / blaCTX-M-15	-	1..137368	137368

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-89)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10577.20 Da
• Isoelectric Point: 9.7617

>T142049 WP_254000279.1 NZ_CP045523:17381-17653 [Shigella flexneri]
MEIFWTMLASQDRKRIRKYIAEQNLMAAIELDERIGYSASSGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVYIL
RVLHTAQKWP

Nucleotide

• Length: 273 bp

>T142049 NZ_CP060388:c1660660-1660367 [Sphingobium sp. KCTC 72723]
GTGTCACGACTGATCTGGACGGCGGAGGCGCTGGCCGATGTTCAGCGGCTCTATCGCTGGTTGTTGCCCAAGGATGCAGA
GGCTGCGAAGCGCGCAGTTGGGGCGATCAGGGCGGGGGTCAGAATATTGGCGCAGCAACCGCGCATCGGTCGCCCGGTAG
AGGATATGGACCCGGAATATCGAGAAAAGCCGATTGATTTCGGGAATAGCGGTTATGTCGTGTTGTATCGCGTCGAGCAG
GATGTCGTGGTGCTGTTGGCCGTGCGGCATCGGAAGGAAGCGGGTTATCCTTAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10252.58 Da
• Isoelectric Point: 7.2057

>AT142049 WP_000079941.1 NZ_CP045523:17112-17381 [Shigella flexneri]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT142049 NZ_CP060388:c1660926-1660648 [Sphingobium sp. KCTC 72723]
ATGGTGACGGCAACATCGATCAAGCTGGACGAGGATATGAAGGGCCGCGTGCAGCATCTGGCAGAGGCGCGTCGTCGCAG
TTCCCACTGGATCATGCGGGAGGCGATTGCGCAATATGTCGAGCGTGAGGAAAAGCGCGAGGCGTTGAAGCAGGATGCCA
TGCAGGCGTGGGAGGGGTATCAGCAGACAGGTCTGCATTTGAGCGTCGATGAGGCCGATAGCTGGCTGGCCAGGCTGGAA
GCCGGTGAGGATGCGGACCTGCCGCCGTGTCACGACTGA

Structures

Antitoxin

• AlphaFold DB: A0A629TXC5