Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1795910..1796705 Replicon chromosome
Accession NZ_CP045522
Organism Shigella flexneri strain 5908.2

Toxin (Protein)


Gene name yeeV Uniprot ID B7UP43
Locus tag GE167_RS08880 Protein ID WP_000854914.1
Coordinates 1796331..1796705 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A7U9LM11
Locus tag GE167_RS08875 Protein ID WP_001280953.1
Coordinates 1795910..1796284 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GE167_RS08840 (1791118) 1791118..1792056 + 939 WP_052961806.1 hypochlorite stress DNA-binding transcriptional regulator HypT -
GE167_RS08845 (1792174) 1792174..1792615 - 442 Protein_1743 IS200/IS605 family transposase -
GE167_RS08850 (1792669) 1792669..1793882 + 1214 WP_136952279.1 IS3 family transposase -
GE167_RS08855 (1793925) 1793925..1794395 + 471 Protein_1745 DUF945 domain-containing protein -
GE167_RS08860 (1794486) 1794486..1794971 + 486 WP_000214398.1 antirestriction protein -
GE167_RS08865 (1794987) 1794987..1795463 + 477 WP_001186738.1 RadC family protein -
GE167_RS08870 (1795526) 1795526..1795747 + 222 WP_000692323.1 DUF987 domain-containing protein -
GE167_RS08875 (1795910) 1795910..1796284 + 375 WP_001280953.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
GE167_RS08880 (1796331) 1796331..1796705 + 375 WP_000854914.1 TA system toxin CbtA family protein Toxin
GE167_RS08885 (1796702) 1796702..1797193 + 492 WP_000976857.1 DUF5983 family protein -
GE167_RS08890 (1797205) 1797205..1797402 + 198 WP_000839282.1 DUF957 domain-containing protein -
GE167_RS08895 (1797487) 1797487..1798329 + 843 WP_053884962.1 DUF4942 domain-containing protein -
GE167_RS08905 (1798800) 1798800..1799738 + 939 Protein_1754 glyoxylate/hydroxypyruvate reductase GhrA -
GE167_RS08910 (1799793) 1799793..1800530 + 738 WP_000283664.1 zinc-binding phosphatase -
GE167_RS08915 (1800554) 1800554..1801108 + 555 WP_001001917.1 molecular chaperone YcdY -
GE167_RS08920 (1801210) 1801210..1801701 + 492 WP_001297187.1 DUF1097 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 14117.17 Da        Isoelectric Point: 7.7761

>T142028 WP_000854914.1 NZ_CP045522:1796331-1796705 [Shigella flexneri]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T142028 NZ_CP060288:4650652-4650948 [Pseudomonas mendocina]
ATGGAAAAGCGCAAACCCCATTTCAAGCTTGAGCTGGTGAAGCAGGCCGTAGCCGACCAGCGTTATCGCTTCACCCGCGT
AGCCTTGGAAGGAGGCGCTGAGCTGGGTATGGAGATGGCCGACATGCTGGCCGTCATCAGTGCCCTGAGCAGCCGCGACT
TTTTCAAGAGCATGACAACCTATGCGGATCATACTACCTGGCAGGACGTTTACCGACCCGATACCGAGTTCGGGCAGGTC
TACCTGAAGTTCACGCTGGTGGCCGATCTGCTGATCGTTTCCTTCAAGGAGAAGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13734.56 Da        Isoelectric Point: 6.6249

>AT142028 WP_001280953.1 NZ_CP045522:1795910-1796284 [Shigella flexneri]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGSCGYVYLAVYPTPALATTS

Download         Length: 375 bp

>AT142028 NZ_CP060288:4650951-4651352 [Pseudomonas mendocina]
ATGAAATGTCCAGTATGCGGCGGGGCGGAACTGGTTCACGACACCCGCGATATGCCTTTCACCTACAAGGGGCAGACCAC
CCAAATTACTGCGGTAACGGCTGACTGGTGCGATGCCTGCGGCGAGTCCCTGACTGGGCCGACTGAGAGTGAGCGCGTTA
TGCGTGCCATGAACGAGTTTCGCCAGCAGGTGAACGCCCAGGACGGCAATCAGGAGCTGATTCGCAGCGTGCGTAAGCAG
TTGCGGTTGAGCCAACGTGAGGCGGCCGAGCTGTTCGGTGGTGGCCCGAATGCGTTTTCTCGTTACGAGCGAGGCAGTAC
GGAAGCGCCGCAGCCGCTGGTGCAGTTGTTCAAGATCCTGGGCCGACACCCGGAGTTGATCAACGAGCTGCGTGCAGGTT
GA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LXR5

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LM11

References