Detailed information of TA system TA142025

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 1093168..1094005
• Replicon: chromosome
• Accession: NZ_CP045522
• Organism: Shigella flexneri strain 5908.2

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: -
• Locus tag: GE167_RS05290
• Protein ID: WP_025758752.1
• Coordinates: 1093168..1093710 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: A0A658YW11
• Locus tag: GE167_RS05295
• Protein ID: WP_005072928.1
• Coordinates: 1093694..1094005 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GE167_RS05270 (1088900)	1088900..1090270	-	1371	WP_119182053.1	MFS transporter	-
GE167_RS05275 (1090664)	1090664..1091614	+	951	WP_225620340.1	tetratricopeptide repeat protein	-
GE167_RS24140 (1091620)	1091620..1091940	+	321	WP_241558678.1	hypothetical protein	-
GE167_RS05280 (1091933)	1091933..1092166	+	234	WP_134800446.1	hypothetical protein	-
GE167_RS05285 (1092177)	1092177..1093112	+	936	WP_024259953.1	tetratricopeptide repeat protein	-
GE167_RS05290 (1093168)	1093168..1093710	-	543	WP_025758752.1	GNAT family N-acetyltransferase	Toxin
GE167_RS05295 (1093694)	1093694..1094005	-	312	WP_005072928.1	DUF1778 domain-containing protein	Antitoxin
GE167_RS05300 (1094190)	1094190..1095080	-	891	WP_025758754.1	heme o synthase	-
GE167_RS05305 (1095092)	1095092..1095421	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
GE167_RS05310 (1095421)	1095421..1096035	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
GE167_RS23800 (1096025)	1096025..1096255	-	231	WP_032327101.1	hypothetical protein	-
GE167_RS05315 (1096261)	1096261..1096934	+	674	Protein_1058	IS66-like element accessory protein TnpA	-
GE167_RS05320 (1096931)	1096931..1097278	+	348	WP_000631709.1	IS66 family insertion sequence element accessory protein TnpB	-
GE167_RS05325 (1097298)	1097298..1098899	+	1602	WP_161550638.1	IS66-like element ISSfl3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1086928..1104743	17815

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (48-155)

Antitoxin

• Vibrio phage ICP1, Orf50 (19-96)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19835.99 Da
• Isoelectric Point: 8.6178

>T142025 WP_025758752.1 NZ_CP045522:c1093710-1093168 [Shigella flexneri]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLER
QRVSGVLQGSQPSEIGVVRLVMLGGARKYQKRGFGQDLLCDFFEHVKKIHQALPIKGVYLDADPATINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T142025 NZ_CP060288:c664292-663723 [Pseudomonas mendocina]
ATGTTCAAGCCGCAGCTGGTGACTTTGCAACGTGGCGCTTTGCGCCTAGAACCGCTCTCCGATGCGGATATCCCGGCGTT
GGTAGCGTTGGCCGAGGCCAATCGCGAAGAGCTGCTGTACATGAGCGGTACCCAGCGGCTGGACTGGTACCGCAGCGCCC
TGGCCGATCTGCGCGAGCAGCGCGCACTGCCGTTCGTCATTCGCCTGGGTGACGAGTTGGTGGGCACCACCCGTTTCGCC
GATTTCATGAACACCCTGCCGGCCTGCGAAATCGGCTGGACCTGGCTCGACCGCAATCAGCATGGCAGCGGCCTGAACAC
CACCATCAAGTACCTGATGCTCAGGCACGCCTTCGACAACTGGGGCATGGTCCGTGTGCAGCTGAAAACCGCTGCCAGCA
ACCAGCGCTCGCAACGTGCCATCGAGAAACTGGGTGCTGTCCGTGAGGGATTGCTGCGCAACCATCGCCGGCTGGCCGAT
GGCCGTCTCGACGATACCGTGCTGTACAGCATCACCGATCTGGAGTGGCCGGCGCTGCGAGAGGCGATGGAGGCGGGCTT
CGGCGCCTGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11410.11 Da
• Isoelectric Point: 10.3388

>AT142025 WP_005072928.1 NZ_CP045522:c1094005-1093694 [Shigella flexneri]
MLGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT142025 NZ_CP060288:c663717-662860 [Pseudomonas mendocina]
ATGCAAGCCCGCGAGCAGACCCGCTTCTGGCAGGCATCCGCGTTGAGCGGAGTCGAGCTGTTGCAGGCTCGCTACCTCGA
ACAACGCTTCGCGCCTCATGTCCATGAGGGCTTCGTCTTCACCGTGATCGAGCACGGTGCGCAGCGTTTTCACCACCGTG
GCAGCGACCACCTGGCGCCACAGGGCAGCATGGTGCTGATCAACCCGGACGAAGTGCACACCGGTTCCAAGGCGCACGAG
GATGGCTGGCGTTATCGCGGCTTCTATCCGGGTAATGATCAGGTGCTGGGGGCGCTGCAGGAACTGGGCCTGGCCAAGAC
GGGCATGCCGTCGTTTTCCTTCAGCGTGCTGCATGATCCGCAGTTGCACAGCGCCTTCCTGCAACTGCATCGCCTGCTCG
ACGGCGGCGCCGAAGCACTGCAGCAGCAACTGGCCTGGCGCGAGGCGATTCTGCTGCTGTTCCAGCGTCACGCCAGGCTT
GCCGAGCCAGCTGCGCCCGGACGTGAGCCCTGGGCCGTAGCCTGTGCCAAGCAGATGCTCGCCGACCGCCTGATCGAGCC
GCCGTCCCTGGAAGAGCTGGCGGCTGCGGTCAACCTTTCACCTTTCCACTTCGCCCGGGTGTTTCGCCGTGCGACCGGTC
TGCCGCCGCACGCATGGCTCAAACAGCGTCGTCTGGAACAGGCGCGGGCGTTGCTCAAGACTGGCTGCACGGCGGTCACC
GTGGCGACGCAGCTGGGTTTTGCCGACCAGAGCCACCTGTCACGACAGTTCAAGCAGGTCTATGGCGTCAGCCCTGGCGA
ATATCGTAGCGCCGTGGCACGGATTGTGGCTGAGGGCGCGCCCCCTGGCGAACGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A658YW11