Detailed information of TA system TA142021

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/MqsA(antitoxin)
• Location: 7051..7544
• Replicon: plasmid pSal-5091_MCR64k
• Accession: NZ_CP045521
• Organism: Salmonella enterica subsp. enterica serovar Anatum strain Sal-5091

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: U9Y9W8
• Locus tag: GE195_RS23500
• Protein ID: WP_001230709.1
• Coordinates: 7051..7305 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: U9Y4R8
• Locus tag: GE195_RS23505
• Protein ID: WP_000151456.1
• Coordinates: 7302..7544 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GE195_RS23465	2628..2789	+	162	WP_001366886.1	type I toxin-antitoxin system Hok family toxin	-
GE195_RS23470	2939..3535	+	597	WP_000125165.1	hypothetical protein	-
GE195_RS23910	3553..3837	-	285	WP_001162371.1	hypothetical protein	-
GE195_RS23480	3911..4576	-	666	WP_000532149.1	P-loop NTPase	-
GE195_RS23485	5165..5494	+	330	WP_001542022.1	hypothetical protein	-
GE195_RS23490	5895..6410	+	516	WP_001025393.1	J domain-containing protein	-
GE195_RS23495	6514..7054	+	541	Protein_7	hypothetical protein	-
GE195_RS23500	7051..7305	+	255	WP_001230709.1	hypothetical protein	Toxin
GE195_RS23505	7302..7544	+	243	WP_000151456.1	hypothetical protein	Antitoxin
GE195_RS23510	8115..8408	+	294	WP_000673428.1	hypothetical protein	-
GE195_RS23515	8897..9145	+	249	WP_000513532.1	type II toxin-antitoxin system HicA family toxin	-
GE195_RS23520	9142..9489	+	348	WP_000681609.1	type II toxin-antitoxin system HicB family antitoxin	-
GE195_RS23525	9577..9759	-	183	WP_001428470.1	hypothetical protein	-
GE195_RS23530	10261..10560	+	300	WP_034169410.1	type II toxin-antitoxin system HicB family antitoxin	-
GE195_RS23535	10820..11146	-	327	WP_000206697.1	hypothetical protein	-
GE195_RS23540	11148..11507	-	360	WP_024147837.1	hypothetical protein	-
GE195_RS23545	11608..12315	-	708	WP_000108794.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..63762	63762

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10912.81 Da
• Isoelectric Point: 12.7242

>T142021 WP_001230709.1 NZ_CP045521:7051-7305 [Salmonella enterica subsp. enterica serovar Anatum]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK
KKKKK

Nucleotide

• Length: 255 bp

>T142021 NZ_CP060283:39486-39791 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGACAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGGCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 10400.12 Da
• Isoelectric Point: 12.7030

>AT142021 WP_000151456.1 NZ_CP045521:7302-7544 [Salmonella enterica subsp. enterica serovar Anatum]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK
K

Nucleotide

• Length: 243 bp

>AT142021 NZ_CP060283:39266-39484 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A3G9H8F0

Antitoxin

• AlphaFold DB: A0A3G9GNF5