Detailed information of TA system TA142014

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF-MazE
• Location: 4104373..4104932
• Replicon: chromosome
• Accession: NZ_CP045508
• Organism: Desulfolutivibrio sulfoxidireducens strain DSM 107105

Toxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: GD604_RS18025
• Protein ID: WP_176638251.1
• Coordinates: 4104373..4104702 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: GD604_RS18030
• Protein ID: WP_176632771.1
• Coordinates: 4104690..4104932 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GD604_RS18010	4100436..4102289	+	1854	WP_176638248.1	4Fe-4S ferredoxin	-
GD604_RS18015	4102365..4103174	+	810	WP_176638249.1	electron transfer flavoprotein subunit beta	-
GD604_RS18020	4103265..4104284	+	1020	WP_176638250.1	electron transfer flavoprotein subunit alpha	-
GD604_RS18025	4104373..4104702	-	330	WP_176638251.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
GD604_RS18030	4104690..4104932	-	243	WP_176632771.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
GD604_RS18035	4105027..4107312	-	2286	WP_176638252.1	response regulator	-
GD604_RS18040	4107779..4108240	-	462	WP_176632773.1	isoprenylcysteine carboxylmethyltransferase family protein	-
GD604_RS18045	4108442..4109347	-	906	WP_176638253.1	DUF1835 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-107)

Antitoxin

• SpoVT-AbrB domain (7-43)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12493.64 Da
• Isoelectric Point: 11.3195

>T142014 WP_176638251.1 NZ_CP045508:c4104702-4104373 [Desulfolutivibrio sulfoxidireducens]
MDVVTPRRFDVFLVGFDPVVGHETAKTRPVIVVSPNELNDSLKTVLVVPLTSTVRNLPFRLRLRFEGRDGEAAPDQMRAV
DARRLVKKLGHLTRRDQDRMLILLREMFA

Nucleotide

• Length: 330 bp

>T142014 NZ_CP060281:4117933-4118151 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8567.68 Da
• Isoelectric Point: 4.5145

>AT142014 WP_176632771.1 NZ_CP045508:c4104932-4104690 [Desulfolutivibrio sulfoxidireducens]
MRATIVSVGNSRGIRIPKAVLEELGSPSSLELRLEGGELVLTPSSVPRQGWAEAARAAASTLEPLPGAPATDWDEKEWTW

Nucleotide

• Length: 243 bp

>AT142014 NZ_CP060281:4117532-4117906 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG